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SUMMARY A β-1,3 glucanase-producing, thermophilic Streptomyces species was grown successfully in a chemically defined medium. Enzyme synthesis was semi-con stitu- tive and subject to catabolite repression by metabolizable carbon substrates; it was inducible by gentiobiose, a molecule structurally unlike its substrate. The enzyme was truly extracellular. Maximum batch production of the glucanase was obtained by adding the inducer late in the fermentation.
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