SUMMARY A membrane-bound sulphite oxidase from uses nitrate, oxygen or ferricyanide as an electron acceptor and is not stimulated by AMP. Studies with inhibitors of oxygen uptake indicated that an electron-transport chain is involved during sulphite oxidation. The inhibition of oxygen uptake by nitrate was found to be of the non-competitive type. The enzyme, which was purified about 50-fold, used ferricyanide as an electron acceptor, but neither nitrate nor oxygen was utilized. Thiol-binding reagents strongly inhibited the enzyme and this was completely reversed by dithiothreitol. The optimum pH was 8.3 with a of 0.5 mM for sulphite.


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