1887

Abstract

SUMMARY A membrane-bound sulphite oxidase from uses nitrate, oxygen or ferricyanide as an electron acceptor and is not stimulated by AMP. Studies with inhibitors of oxygen uptake indicated that an electron-transport chain is involved during sulphite oxidation. The inhibition of oxygen uptake by nitrate was found to be of the non-competitive type. The enzyme, which was purified about 50-fold, used ferricyanide as an electron acceptor, but neither nitrate nor oxygen was utilized. Thiol-binding reagents strongly inhibited the enzyme and this was completely reversed by dithiothreitol. The optimum pH was 8·3 with a of 0·5 m for sulphite.

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1974-05-01
2021-07-31
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References

  1. Adair F. W. 1966; Membrane-associated sulphur oxidation by the autotroph Thiobacillus thio-oxidans . Journal of Bacteriology 92:899–904
    [Google Scholar]
  2. Adams C. A., Warnes G. M., Nicholas D. J. D. 1971; A sulphite-dependent nitrate reductase from Thiobacillus denitrificans . Biochimica et biophysica acta 235:398–406
    [Google Scholar]
  3. Aminuddin M., Nicholas D. J. D. 1973; Sulphide oxidation linked to the reduction of nitrate and nitrite in Thiobacillus denitrificans . Biochimica et biophysica acta 325:81–93
    [Google Scholar]
  4. Aminuddin M., Nicholas D. J. D. 1974; Electron transfer during sulphide and sulphite oxidation in Thiobacillus denitrificans . Journal of General Microbiology 82:115–123
    [Google Scholar]
  5. Bowen T. J., Happold F. C., Taylor B. F. 1966; Studies on adenosine 5ʹ-phosphosulphate reductase from Thiobacillus denitrificans . Biochimica et biophysica acta 118:566–576
    [Google Scholar]
  6. Charles A. M., Suzuki I. 1966a; Mechanism of thiosulphate oxidation by Thiobacillus novellus . Biochimica et biophysica acta 128:510–521
    [Google Scholar]
  7. Charles A. M., Suzuki I. 1966b; Purification and properties of sulphite: cytochrome c oxido-reductase from Thiobacillus novellus . Biochimica et biophysica acta 128:522–534
    [Google Scholar]
  8. Dixon M., Kleppe K. 1965; d-Amino acid oxidase. I. Dissociation and recombination of the holo-enzyme. Biochimica et biophysica acta 96:357–367
    [Google Scholar]
  9. Hempfling W. P., Trudinger P. A., Vishniac W. 1967; Purification and some properties of sulphite oxidase from Thiobacillus neapolitanus . Archiv für Mikrobiologie 59:149–157
    [Google Scholar]
  10. Hewitt E.J., Nicholas D. J. D. 1968; Enzymes of inorganic nitrogen metabolism. In Modern Methods of Plant Analysis 7 pp 67–172 Linskens Y. F., Sanwal B. D., Tracey M. V. Edited by Berlin: Springer Verlag;
    [Google Scholar]
  11. Kodama A., Mori T. 1968; Studies on the metabolism of a sulphur oxidizing bacterium. V. Comparative studies of sulphur and sulphite oxidizing systems in Thiobacillus thio-oxidans . Plant and Cell Physiology 9:725–734
    [Google Scholar]
  12. London J., Rittenberg S. G. 1964; Path of S in sulphide and thiosulphate oxidation by Thiobacilli . Proceedings of the National Academy of Sciences of the United States of America 52:1183–1190
    [Google Scholar]
  13. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  14. Lyric M. R., Suzuki I. 1970a; Enzymes involved in the metabolism of thiosulphate by Thiobacillus thioparus. I. Survey of enzymes and properties of sulphite:cytochrome c oxido-reductase. Canadian Journal of Biochemistry 48:334–343
    [Google Scholar]
  15. Lyric M. R., Suzuki I. 1970b; Enzymes involved in the metabolism of thiosulphate by Thiobacillus thioparus. II. Properties of APS reductase. Canadian Journal of Biochemistry 48:344–353
    [Google Scholar]
  16. Lyric M. R., Suzuki I. 1970c; Enzymes involved in the metabolism of thiosulphate by Thiobacillus thioparus. III. Properties of thiosulphate oxidizing enzyme and proposed pathway of thiosulphate oxidation. Canadian Journal of Biochemistry 48:355–363
    [Google Scholar]
  17. Moriarty D. J. W. 1969 Inorganic sulphur compound oxidation in Thiobacillus concretivorus Ph. D. thesis University of Adelaide:
    [Google Scholar]
  18. Moriarty D. J. W., Nicholas D. J. D. 1970; Electron transfer during sulphide and sulphite oxidation by Thiobacillus concretivorus . Biochimica et biophysica acta 216:130–138
    [Google Scholar]
  19. Peck H. D. Jun 1960; Adenosine 5ʹ-phosphosulphate as an intermediate in the oxidation of thiosulphate by Thiobacillus thioparus . Proceedings of the National Academy of Sciences of the United States of America 46:1053–1057
    [Google Scholar]
  20. Peck H. D. Jun 1962; Symposium on metabolism of inorganic compounds. V. Comparative metabolism of inorganic sulphur compounds in micro-organisms. Bacteriological Reviews 26:67–94
    [Google Scholar]
  21. Peck H. D. Jun Deacon T. E., Davidson J. T. 1966; Studies on APS reductase from Desulpho-vibrio desulphuricans and Thiobacillus thioparus. I. The assay and purification. Biochimica et biophysica acta 96:429–446
    [Google Scholar]
  22. Trudinger P. A. 1967; The metabolism of inorganic sulphur compounds by thiobacilli. Review of Pure and Applied Chemistry 17:1–24
    [Google Scholar]
  23. Trudinger P. A. 1969; Assimilatory and dissimilatory metabolism of inorganic sulphur compounds by micro-organisms. In Advances in Microbial Physiology 3 pp 111–158 Rose A. H., Wilkinson J. F. Edited by New York: Academic Press;
    [Google Scholar]
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