@article{mbs:/content/journal/micro/10.1099/00221287-82-1-103, author = "Aminuddin, M. and Nicholas, D. J. D.", title = "An AMP-independent Sulphite Oxidase from Thiobacillus denitrificans: Purification and Properties", journal= "Microbiology", year = "1974", volume = "82", number = "1", pages = "103-113", doi = "https://doi.org/10.1099/00221287-82-1-103", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-82-1-103", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY A membrane-bound sulphite oxidase from Thiobacillus denitrificans uses nitrate, oxygen or ferricyanide as an electron acceptor and is not stimulated by AMP. Studies with inhibitors of oxygen uptake indicated that an electron-transport chain is involved during sulphite oxidation. The inhibition of oxygen uptake by nitrate was found to be of the non-competitive type. The enzyme, which was purified about 50-fold, used ferricyanide as an electron acceptor, but neither nitrate nor oxygen was utilized. Thiol-binding reagents strongly inhibited the enzyme and this was completely reversed by dithiothreitol. The optimum pH was 8·3 with a Km of 0·5 mm for sulphite.", }