1887

Abstract

The pathway of glucose metabolism in was studied by a radiorespirometric technique and assays of enzyme activity in cell-free extracts. The primary catabolic pathways in the organism were examined by measurement of relative rates of CO-production from different carbon atoms of labelled glucose, pyruvic acid and acetic acid. These substrates appeared to be degraded solely by enzymes of the Embden-Meyerhof-Parnas pathway in conjunction with the tricarboxylic acid cycle. The results were confirmed by studies of enzyme activity, which showed a lack of two enzymes, glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate dehydrogenase, EC. 1.1.1.49) and 6-phospho-gluconate dehydrogenase [6-phospho-D-gluconate: NADP oxidoreductase (decarboxylating), EC. 1.1.1.44], in the pentose pathway, which indicated a biosynthetic function of the non-oxidative part of this pathway.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-81-1-27
1974-03-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/81/1/mic-81-1-27.html?itemId=/content/journal/micro/10.1099/00221287-81-1-27&mimeType=html&fmt=ahah

References

  1. Bowman J. E., Brubaker R. R., Frischer H., Carson P. E. 1967; Characterization of enterobacteria by starch-gel electrophoresis of glucose-6-phosphate dehydrogenase and phosphogluconate dehydrogenase. Journal of Bacteriology 94:544–551
    [Google Scholar]
  2. Brubaker R. R. 1968; Metabolism of carbohydrates by Pasteurella pseudotuberculosis. Journal of Bacteriology 95:1698–1705
    [Google Scholar]
  3. Fåhreus G. 1947; Studies in the cellulose decomposition by Cytophaga. Symbolae botanicae upsalienses IX:2–128
    [Google Scholar]
  4. Fraenkel D. G. 1968; Selection of Escherichia coli mutants lacking glucose-6-phosphate dehydrogenase or gluconate-6-phosphate dehydrogenase. Journal of Bacteriology 95:1267–1271
    [Google Scholar]
  5. Gurvin I. 1968 Anvendelse av radio-Warburgteknikk til måling av respirasjon og fotosyntese Cand. real, thesis University of Bergen, Norway:
    [Google Scholar]
  6. Hespell R. B., Canale-Parola E. 1970; Carbohydrate metabolism in Spirochaeta stenostrepta. Journal of Bacteriology 103:216–226
    [Google Scholar]
  7. Horecker B. L., Smyrniotis P. Z. 1955; 6-Phosphogluconic acid dehydrogenase. In Methods in Enzymology 1 pp. 323–327 Colowick S. P., Kaplan N. O. Edited by New York: Academic Press;
    [Google Scholar]
  8. Keele B. B.Jun Hamilton P. B., Elkan G. H. 1970; Gluconate catabolism in Rhizobium japonicum. Journal of Bacteriology 101:698–704
    [Google Scholar]
  9. Kornberg A., Horecker B. L. 1955; Glucose-6-phosphate dehydrogenase. In Methods in Enzymology 1 pp. 323–327 Colowick S. P., Kaplan N. O. Edited by New York: Academic Press;
    [Google Scholar]
  10. Linke H. A. B. 1969; Fructose metabolism in Clostridium aceticum. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene Abteilung II 123:369–379
    [Google Scholar]
  11. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  12. Mahler H. R., Cordes E. H. 1969 Biological Chemistry New York: Harper and Row;
    [Google Scholar]
  13. Raa J., Goksøyr J. 1965; Studies on the effects of the heartwood toxin β-thujaplicin on the metabolism of yeast. Physiologia plantarum 18:159–176
    [Google Scholar]
  14. Raj H. D. 1967; Radiorespirometric studies of Leucothrix mucor. Journal of Bacteriology 94:615–623
    [Google Scholar]
  15. Speyer E. 1953; Untersuchungen an Sporocytophaga myxococcoides (Stanier 1940). Archiv für Mikrobiologie 18:245–272
    [Google Scholar]
  16. Stanier R. Y. 1940; Studies on the Cytophagas. Journal of Bacteriology 40:619–635
    [Google Scholar]
  17. Stanier R. Y. 1942; The Cytophaga group. A contribution to the biology of Myxobacteria. Bacteriological Reviews 6:143–196
    [Google Scholar]
  18. Starr T. J., Klein H. P. 1954; Enzymes involved in the utilization of carbohydrates by two strains of myxobacteria. Archiv für Mikrobiologie 20:235–242
    [Google Scholar]
  19. Umbreit W. W., Burris R. H., Stauffer J. F. 1957 Manometric Techniques Minneapolis: Burgess Publishing;
    [Google Scholar]
  20. Verma J. P., Martin H. H. 1967; Über die Oberflächenstruktur von Myxobacterien. I. Chemie und Morphologie der Zellwände von Cytophaga hutchinsonii und Sporocytophaga myxococcoides. Archiv für Mikrobiologie 59:355–380
    [Google Scholar]
  21. Wang C. H. 1972; Radiorespirometric methods. In Methods in Microbiology pp. 185–230 Norris J. R., Ribbons D. W. Edited by London and New York: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-81-1-27
Loading
/content/journal/micro/10.1099/00221287-81-1-27
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error