1887

Abstract

The pathway of glucose metabolism in was studied by a radiorespirometric technique and assays of enzyme activity in cell-free extracts. The primary catabolic pathways in the organism were examined by measurement of relative rates of CO-production from different carbon atoms of labelled glucose, pyruvic acid and acetic acid. These substrates appeared to be degraded solely by enzymes of the Embden-Meyerhof-Parnas pathway in conjunction with the tricarboxylic acid cycle. The results were confirmed by studies of enzyme activity, which showed a lack of two enzymes, glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate dehydrogenase, EC. 1.1.1.49) and 6-phospho-gluconate dehydrogenase [6-phospho-D-gluconate: NADP oxidoreductase (decarboxylating), EC. 1.1.1.44], in the pentose pathway, which indicated a biosynthetic function of the non-oxidative part of this pathway.

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/content/journal/micro/10.1099/00221287-81-1-27
1974-03-01
2019-10-21
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-81-1-27
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