The pathway of glucose metabolism in was studied by a radiorespirometric technique and assays of enzyme activity in cell-free extracts. The primary catabolic pathways in the organism were examined by measurement of relative rates of CO-production from different carbon atoms of labelled glucose, pyruvic acid and acetic acid. These substrates appeared to be degraded solely by enzymes of the Embden-Meyerhof-Parnas pathway in conjunction with the tricarboxylic acid cycle. The results were confirmed by studies of enzyme activity, which showed a lack of two enzymes, glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate dehydrogenase, EC. and 6-phospho-gluconate dehydrogenase [6-phospho-D-gluconate: NADP oxidoreductase (decarboxylating), EC.], in the pentose pathway, which indicated a biosynthetic function of the non-oxidative part of this pathway.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error