SUMMARY: In Sitka spruce nursery plots the numbers of amoebae in steam-treated soil rose with the increase in the bacterial population. The population of amoebae over a period of seven months was much higher in this soil than in the untreated or the formalin-treated soils. The formalin-treated soil had significantly lower numbers of amoebae compared with the untreated soil over a period of one year, although the bacterial numbers were often higher in the former than in the latter. It is suggested that the unsuitable quality of bacterial food supply might be responsible for keeping the numbers of amoebae in check in the formalin-treated soil. Double formalin treatment seemed to suppress further the numbers of amoebae.


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