SUMMARY: The growth of large quantities of a TRIC agent and the concomitant production of polysaccharide were studied in BHK21 and L cell cultures in suspension. The maximum concentrations of elementary bodies and polysaccharide/culture were attained at about the same time (60 h) after infection in both cell systems. The yield of elementary bodies from a 11 culture was equivalent to the maximum obtained from a total area of 8000 cm of BHK21 monolayers. Polysaccharide found in the medium was probably derived from disrupted inclusion bodies. It is postulated that synthesis of the polysaccharide is directed by the TRIC agent but controlled by the host cell.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error