1887

Abstract

SUMMARY: The growth of large quantities of a TRIC agent and the concomitant production of polysaccharide were studied in BHK21 and L cell cultures in suspension. The maximum concentrations of elementary bodies and polysaccharide/culture were attained at about the same time (60 h) after infection in both cell systems. The yield of elementary bodies from a 11 culture was equivalent to the maximum obtained from a total area of 8000 cm of BHK21 monolayers. Polysaccharide found in the medium was probably derived from disrupted inclusion bodies. It is postulated that synthesis of the polysaccharide is directed by the TRIC agent but controlled by the host cell.

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/content/journal/micro/10.1099/00221287-78-2-297
1973-10-01
2022-01-22
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