1887

Abstract

SUMMARY: A polygalacturonase has been purified from the culture fluid of a strain of . The purified preparation was homogeneous when examined by ultracentrifugation. The hydrolysis of di- and trigalacturonic acids by the enzyme was undetectable. Tetragalacturonic acid was hydrolysed to tri- and monogalacturonic acids, while pentagalacturonic acid was hydrolysed to either tri- and digalacturonic acids or tetra- and monogalacturonic acids. The rate of hydrolysis of pentagalacturonic acid to tri- and digalacturonic acids was about four times as fast as that to tetra- and monogalacturonic acids. It was concluded that the enzyme can hydrolyse any glycosidic bond between the reducing end and the third galacturonic-acid unit from the non-reducing end of poly-galacturonic acid. The manner of hydrolysis of polygalacturonic acid by the enzyme is different from that of any other polygalacturonase reported so far.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-74-1-127
1973-01-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/74/1/mic-74-1-127.html?itemId=/content/journal/micro/10.1099/00221287-74-1-127&mimeType=html&fmt=ahah

References

  1. Demain A. L., Phaff H. J. 1957; Recent advances in the enzymatic hydrolysis of pectic substances. Wallerstein Laboratories Communications 20:119–140
    [Google Scholar]
  2. Dische Z. 1947; A new specific color reaction of Hexuronic acids. Journal of Biological Chemistry 167:189–198
    [Google Scholar]
  3. Endo A. 1964a; Studies on pectolytic enzymes of molds: Part VIII. Purification and properties of endo-polygalacturonase I. Agricultural and Biological Chemistry 28:535–542
    [Google Scholar]
  4. Endo A. 1964b; Studies on pectolytic enzymes of molds: Part XI. Purification and properties of exopolygalacturonase. Agricultural and Biological Chemistry 28:639–645
    [Google Scholar]
  5. Hatanaka C. 1967; Micro-iodometry of aldose. Journal of the Agricultural Chemical Society of Japan 41:448–453
    [Google Scholar]
  6. Hatanaka C., Ozawa J. 1966; Enzymic degradation of pectic acid. Journal of the Agricultural Chemical Society of Japan 40:98–105
    [Google Scholar]
  7. Jansen E. F., MacDonnell L. R. 1945; Influence of methoxyl content of pectic substances on the action of polygalacturonase. Archives of Biochemistry 8:97–112
    [Google Scholar]
  8. Koller A., Neukom H. 1969; Untersuchungen über den Abbaumechanismus einer gereinigten Polygalacturonase aus Aspergillus niger. European Journal of Biochemistry 7:485–489
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Matsumura T. 1969; Chemistry of constituents of polysaccharides. In Tatō seikagaku vol 1 p 53 Edited by Suzuki A., Matsumura T., Yamashina I. Tokyo: Kyoritsu Press;
    [Google Scholar]
  11. Mill P. J. 1966a; The pectic enzymes of Aspergillus niger: a mercury-activated exopolygalacturonase. Biochemical Journal 99:557–561
    [Google Scholar]
  12. Mill P. J. 1966b; The pectic enzymes of Aspergillus niger: a second exopolygalacturonase. Biochemical Journal 99:562–565
    [Google Scholar]
  13. Mill P. J., Tuttobello R. 1961; The pectic enzymes of Aspergillus niger: 2. Endopolygalacturonase. Biochemical Journal 79:57–64
    [Google Scholar]
  14. Nasuno S., Starr M. P. 1966; Polygalacturonase of Erwinia carotovora. Journal of Biological Chemistry 241:5298–5306
    [Google Scholar]
  15. Pásková J., Munk V. 1960; A combined detecting reagent for the identification of organic acids on paper chromatograms. Journal of Chromatography 4:241–243
    [Google Scholar]
  16. Patel D. S., Phaff H. J. 1959; On the mechanism of action of yeast endo-polygalacturonase on oligogalacturonides and their reduced and oxidized derivatives. Journal of Biological Chemistry 234:237–241
    [Google Scholar]
  17. Patel D. S., Phaff H. J. 1960; Properties of purified tomato polygalacturonase. Food Research 25:47–57
    [Google Scholar]
  18. Phaff H. J., Luh B. S. 1952; The preparation of pure di- and trigalacturonic acids. Archives of Biochemistry and Biophysics 36:231–232
    [Google Scholar]
  19. Preiss J., Ashwell G. 1963; Polygalacturonic acid metabolism in bacteria: II. Formation and metabolism of 3-deoxy–d-glycero-2,5-hexodiulosonic acid. Journal of Biological Chemistry 238:1577–1583
    [Google Scholar]
  20. Roboz E., Barratt R. W., Tatum E. L. 1952; Breakdown of pectic substances by a new enzyme from Neurospora. Journal of Biological Chemistry 195:459–471
    [Google Scholar]
  21. Uchino F., Kurono Y., Doi S. 1966; Crystallization of an endopolygalacturonase from Acrocylindrium sp. Agricultural and Biological Chemistry 30:1066–1068
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-74-1-127
Loading
/content/journal/micro/10.1099/00221287-74-1-127
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error