@article{mbs:/content/journal/micro/10.1099/00221287-74-1-127, author = "Kimura, H. and Uchino, F. and Mizushima, S.", title = "Properties of a Polygalacturonase Produced by Acrocylindrium", journal= "Microbiology", year = "1973", volume = "74", number = "1", pages = "127-137", doi = "https://doi.org/10.1099/00221287-74-1-127", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-74-1-127", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: A polygalacturonase has been purified from the culture fluid of a strain of Acrocylindrium. The purified preparation was homogeneous when examined by ultracentrifugation. The hydrolysis of di- and trigalacturonic acids by the enzyme was undetectable. Tetragalacturonic acid was hydrolysed to tri- and monogalacturonic acids, while pentagalacturonic acid was hydrolysed to either tri- and digalacturonic acids or tetra- and monogalacturonic acids. The rate of hydrolysis of pentagalacturonic acid to tri- and digalacturonic acids was about four times as fast as that to tetra- and monogalacturonic acids. It was concluded that the enzyme can hydrolyse any glycosidic bond between the reducing end and the third galacturonic-acid unit from the non-reducing end of poly-galacturonic acid. The manner of hydrolysis of polygalacturonic acid by the enzyme is different from that of any other polygalacturonase reported so far.", }