SUMMARY: Growth yields of from carbon source and oxygen were reassessed to define more clearly the terms, maintenance and true growth yield.

In oxygen-limited chemostat cultures, the maintenance coefficients, (mmol glucose/mg bacteria/h) and (mmol O/mg bacteria/h) were 8 × 10 and 5.5 × 10; i.e. 6 . This relationship between and explains the perfect oxidation of carbohydrate (glucose) by maintenance metabolism. Values of the true growth yield, (mg bacteria/mmol glucose) and (mg bacteria/mmol O) were 46 and 13; these values were lower than those recorded for other micro-organisms.

In glucose-limited chemostat cultures, fairly high values of and were observed and in addition, negative values of and were obtained. Energy-uncoupled growth could account for these negative values.


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