1887

Abstract

SUMMARY: Growth yields of from carbon source and oxygen were reassessed to define more clearly the terms, maintenance and true growth yield.

In oxygen-limited chemostat cultures, the maintenance coefficients, (mmol glucose/mg bacteria/h) and (mmol O/mg bacteria/h) were 8 × 10 and 5.5 × 10; i.e. 6 . This relationship between and explains the perfect oxidation of carbohydrate (glucose) by maintenance metabolism. Values of the true growth yield, (mg bacteria/mmol glucose) and (mg bacteria/mmol O) were 46 and 13; these values were lower than those recorded for other micro-organisms.

In glucose-limited chemostat cultures, fairly high values of and were observed and in addition, negative values of and were obtained. Energy-uncoupled growth could account for these negative values.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-73-3-531
1972-12-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/73/3/mic-73-3-531.html?itemId=/content/journal/micro/10.1099/00221287-73-3-531&mimeType=html&fmt=ahah

References

  1. Ackrell B. A. C., Jones C. W. 1971; The respiratory system of Azotobacter vinelandii. 2. Oxygen effects. European Journal of Biochemistry 20:29–35
    [Google Scholar]
  2. Ackrell B. A. C., Jones C. W. 1971; The respiratory system of Azotobacter vinelandii. 2. Oxygen effects. European Journal of Biochemistry 20:29–35
    [Google Scholar]
  3. Bauchop T., Elsden S. R. 1960; The growth of micro-organisms in relation to their energy supply. Journal of General Microbiology 23:457–469
    [Google Scholar]
  4. Hadjipetrou L. P., Gerrits J. P., Teulings F. A. G., Stouthamer A. H. 1964; Relation between energy production and growth of Aerobacter aerogenes . Journal of General Microbiology 36:139–150
    [Google Scholar]
  5. Harrison D. E. F., Loveless J. E. 1971; The effect of growth conditions on respiratory activity and growth efficiency in facultative anaerobes grown in chemostat culture. Journal of General Microbiology 68:35–43
    [Google Scholar]
  6. Herbert D. 1958; Some principles of continuous culture. Symposium of the International Congress of Microbiology, no. 6 pp. 381–396
    [Google Scholar]
  7. Hernandez E., Johnson M. J. 1967a; Anaerobic growth yield of Aerobacter cloacae and Escherichia coli . Journal of Bacteriology 94:991–995
    [Google Scholar]
  8. Hernandez E., Johnson M. J. 1967b; Energy supply and cell yield in aerobically grown micro-organisms. Journal of Bacteriology 94:996–1001
    [Google Scholar]
  9. Hobson P. N. 1965; Continuous culture of some anaerobic and facultatively anaerobic rumen bacteria. Journal of General Microbiology 38:167–180
    [Google Scholar]
  10. Johnson M. J. 1964; Utilisation of hydrocarbons by microorganisms. Chemistry and Industry,1532–1537
    [Google Scholar]
  11. Marr A. G., Nilson E. H., Clark D. J. 1963; The maintenance requirement of Escherichia coli . Annals of the New York Academy of Sciences 102:536–548
    [Google Scholar]
  12. von Meyenburg H. K. 1969; Energetics of the budding cycle of Saccharomyces cerevisiae during glucose limited aerobic growth. Archiv für Mikrobiologie 66:289–303
    [Google Scholar]
  13. Nagai S., Aiba S. 1972; Kinetics of yield factor as affected by dissolved oxygen in a chemostat culture of Azotobacter vinelandii . Symposium of International Fermentation, no. 4 (in the press)
    [Google Scholar]
  14. Nagai S., Nishizawa Y., Aiba S. 1969; Energetics of growth of Azotobacter vinelandii in a glucose-limited chemostat culture. Journal of General Microbiology 59:163–169
    [Google Scholar]
  15. Nagai S., Nishizawa Y., Endo L., Aiba S. 1968; A sophisticated analysis of yield factors for Azotobacter vinelandii . Journal of Fermentation Technology 46:725–732
    [Google Scholar]
  16. Nagai S., Nishizawa Y., Onodera M., Aiba S. 1971; Effect of dissolved oxygen on growth yield and aldolase activity in chemostat culture of Azotobacter vinelandii . Journal of General Microbiology 66:197–203
    [Google Scholar]
  17. Payne W. J. 1970; Energy yield and growth of heterotrophs. Annual Review of Microbiology 24:17–51
    [Google Scholar]
  18. Phillips D. H., Johnson M. J. 1961; Aeration in fermentations. Journal of Biochemical and Microbiological Technology and Engineering 3:277–309
    [Google Scholar]
  19. Pirt S. J. 1965; The maintenance energy of bacteria in growing cultures. Proceedings of The Royal Society B 163:224–231
    [Google Scholar]
  20. Righelato R. C. R., Trinci A. P. J., Pirt S. J., Peat A. 1968; The influence of maintenance energy and growth rate on the metabolic activity, morphology and conidiation of Penicillium chrysogenum . Journal of General Microbiology 50:399–412
    [Google Scholar]
  21. Schultze K. L., Lipe R. S. 1964; Relationship between substrate concentration, growth rate, and respiration rate of Escherichia coli in continuous culture. Archiv für Mikrobiologie 48:1–20
    [Google Scholar]
  22. Senez J. C. 1962; Some considerations on the energetics of bacterial growth. Bacteriological Reviews 26:95–107
    [Google Scholar]
  23. Stouthamer A. H. 1962; Energy production in Gluconobacter liquefaciens . Biochimica et biophysica acta 56:19–32
    [Google Scholar]
  24. van Uden N. 1968; Yield and maintenance analysis in the chemostat; a tool for metabolic studies of growing cells. Archiv für Mikrobiologie 62:34–40
    [Google Scholar]
  25. Waley S. G. 1964; A note of the kinetics of multi-enzyme system. Biochemical Journal 91:514–517
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-73-3-531
Loading
/content/journal/micro/10.1099/00221287-73-3-531
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error