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SUMMARY: Growth yields of Azotobacter vinelandii from carbon source and oxygen were reassessed to define more clearly the terms, maintenance and true growth yield.
In oxygen-limited chemostat cultures, the maintenance coefficients, m (mmol glucose/mg bacteria/h) and m 0 (mmol O2/mg bacteria/h) were 8 × 10−4 and 5.5 × 10−3; i.e. 6m ≃ m 0. This relationship between m and m 0 explains the perfect oxidation of carbohydrate (glucose) by maintenance metabolism. Values of the true growth yield, Y G (mg bacteria/mmol glucose) and Y GO (mg bacteria/mmol O2) were 46 and 13; these values were lower than those recorded for other micro-organisms.
In glucose-limited chemostat cultures, fairly high values of m and m 0 were observed and in addition, negative values of Y G and Y GO were obtained. Energy-uncoupled growth could account for these negative values.
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