1887

Abstract

SUMMARY

PS18(RP1) is resistant to carbenicillin, neomycin/kanamycin and tetracycline. This strain gave rise to a derivative: PS18(RP1-1), resistant only to carbenicillin. RP1 was transmissible from PS18(RP1) to other strains of or while RP1-1 was transmissible to other strains of could not be transferred to and transferred only at a very low frequency to DNA hybridization showed that PS18(RP1-1) contained approximately one sixth as much RP1-homologous DNA as PS18(RP1). Although satellite DNA could be detected in strains carrying RP1 by ethidium bromide/CsCl-gradient analysis and by analytical CsCl-gradient centrifuging, no such satellite DNA could be found in strains of carrying RP1-1 or carrying a non-transferable derivative RP1-1. Drug resistance could be eliminated from RP1-containing strains by treatment with sodium dodecyl sulphate, but the carbenicillin resistance of RP1-1 strains could not be eliminated. The apparent absence of satellite DNA in bacteria carrying RP1-1 or RP1-1, and the failure to ‘cure’ them of their carbenicillin resistance suggests the possibility that the RP1-1 and RP1-1 genes might be a fragment of RP1 integrated in the chromosome of the strains of containing RP1-1 and containing RP1-1.

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/content/journal/micro/10.1099/00221287-72-2-269
1972-09-01
2022-01-23
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References

  1. ANDERSON E. S. 1969 Ecology and epidemiology of transferable drug resistance.. In Bacterial Episomes and Plasmids. Edited by Wolstenholme G. E. W., O’Connor M. London: Ciba Foundation Symposium. J. and A. Churchill;
    [Google Scholar]
  2. CLEWELL D. B., HELSINKI D. R. 1970; Properties of a supercoiled deoxyribonucleic acid - protein relaxation complex and strand specificity of the relaxation event.. Biochemistry 9:4428–4439
    [Google Scholar]
  3. DENHARDT D. T. 1966; A membrane filter technique for the detection of complementary DNA.. Biochemical and Biophysical Research Communications 33:641–646
    [Google Scholar]
  4. FALKOW S., HAAPALA D. K., SILVER R. P. 1969 Relationships between extrachromosomal elements.. In Bacterial Episomes and Plasmids. Edited by Wolstenholme G. E. W., O’Connor M. London: Ciba Foundation Symposium. J. and A. Churchill;
    [Google Scholar]
  5. GRINSTED J., SAUNDERS J. R., INGRAM L. C., SYKES R. B., RICHMOND M. H. 1972; Properties of an R-factor from Pseudomonas aeruginosa 1822.. Journal of Bacteriology In the press
    [Google Scholar]
  6. KONTOMICHALOU P., MITANI M., CLOWES R. C. 1970; Circular R-factor molecules controlling penicillinase synthesis, replicating in Escherichia coli under either relaxed or stringent control.. Journal of Bacteriology 104:34–44
    [Google Scholar]
  7. MARMUR J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms.. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  8. NOVICK R. P. 1969; Extrachromosomal inheritance in bacteria.. Bacteriological Reviews 33:210–235
    [Google Scholar]
  9. Rownd R., NAKAYA R., NAKAMURA A. 1966; Molecular nature of the drug resistance factors of the Enterobacteriaceae.. Journal of Molecular Biology 17:376–393
    [Google Scholar]
  10. SILVER R. P., FALKOW S. 1970; Specific labelling and physical characterisation of R-factor DNA in Escherichia coli.. Journal of Bacteriology 104:34–44
    [Google Scholar]
  11. STANISICH V. A., HOLLOWAY B. W. 1971; Chromosome transfer in Pseudomonas aeruginosa mediated by R-factors.. Genetics Research 17:169–172
    [Google Scholar]
  12. TOMOEDA M., INUZUKA M., KUBO N., NAKAMURA S. 1968; Effective elimination of drug resistance and sex factors in Escherichia coli by sodium dodecyl sulphate.. Journal of Bacteriology 95:1078–1089
    [Google Scholar]
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