1887

Abstract

SUMMARY: PS18(RP1) is resistant to carbenicillin, neomycin/kanamycin and tetracycline. This strain gave rise to a derivative: PS18(RP1-1), resistant only to carbenicillin. RP1 was transmissible from PS18(RP1) to other strains of or while RP1-1 was transmissible to other strains of could not be transferred to and transferred only at a very low frequency to DNA hybridization showed that PS18(RP1-1) contained approximately one sixth as much RP1-homologous DNA as PS18(RP1). Although satellite DNA could be detected in strains carrying RP1 by ethidium bromide/CsCl-gradient analysis and by analytical CsCl-gradient centrifuging, no such satellite DNA could be found in strains of carrying RP1-1 or carrying a non-transferable derivative RP1-1. Drug resistance could be eliminated from RP1-containing strains by treatment with sodium dodecyl sulphate, but the carbenicillin resistance of RP1-1 strains could not be eliminated. The apparent absence of satellite DNA in bacteria carrying RP1-1 or RP1-1, and the failure to ‘cure’ them of their carbenicillin resistance suggests the possibility that the RP1-1 and RP1-1 genes might be a fragment of RP1 integrated in the chromosome of the strains of containing RP1-1 and containing RP1-1.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-72-2-269
1972-09-01
2019-10-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-72-2-269
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error