1887

Abstract

SUMMARY: was efficiently eliminated by acridine orange from 12, whether sex pilus synthesis was constitutive or repressed by an R factor. R factors, F-like or I-like, repressed or derepressed, were not eliminated by acridine orange. Sodium dodecyl sulphate (SDS), known to eliminate certain plasmids determining constitutive synthesis of F pili, had no effect on cultures with wild-type (repressed) R factors. Bacteria with derepressed synthesis of pili, either F-like or I-like, but particularly the latter, showed increased sensitivity to SDS. SDS treatment selected from such cultures non-piliated cells, with lost or mutant R factors. Thus acridine orange was a true ‘curing’ agent, specific for F, whilst SDS acted only by selection of spontaneous variants.

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/content/journal/micro/10.1099/00221287-70-3-443
1972-05-01
2021-10-23
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