1887

Abstract

SUMMARY: organisms recovered from aerosols had a changed envelope as manifested by the bacteria becoming sensitive to lysozyme. Certain di- and trivalent cations added to suspensions of bacteria recovered from aerosols mitigated this sensitivity. Repair required a carbon-energy source, but not synthesis of protein, ribonucleic acid or mucopeptide. Components of the envelope were released from the aerosolized organisms. These results support the hypothesis that aerosolization caused changes in the outer wall structure of bacteria that resemble changes induced by ethylenediaminetetraacetic acid.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-69-1-81
1971-11-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/69/1/mic-69-1-81.html?itemId=/content/journal/micro/10.1099/00221287-69-1-81&mimeType=html&fmt=ahah

References

  1. Anderson J. D., Dark F. A., Peto S. 1968; The effect of aerosolization upon survival and potassium retention by various bacteria. Journal of General Microbiology 52:99–105
    [Google Scholar]
  2. Asbell M. A., Eagon R. G. 1966; The role of multivalent cations in the organisation and structure of bacterial cell walls. Biochemical and Biophysical Research Communications 22:664–671
    [Google Scholar]
  3. Benbough J. E. 1967; Death mechanisms in airborne Escherichia coli. Journal of General Microbiology 47:325–333
    [Google Scholar]
  4. Cox C. S. 1968; A method for the routine preservation of micro-organisms. Nature; London: 2201139
    [Google Scholar]
  5. Cox C. S. 1969; The cause of loss of viability of airborne Escherichia coli k 12. Journal of General Microbiology 57:77–80
    [Google Scholar]
  6. Gel’man N. S., Lukoyanova M. A., Ostrovskii D. N. 1967 Respiration and Phosphorylation of Bacteria New York: Plenum Press;
    [Google Scholar]
  7. Green H. L., Lane W. R. 1957 Particulate Clouds: Dusts, Smokes and Mists London: E. and F. N. Spon;
    [Google Scholar]
  8. Hambleton P. 1970; The sensitivity of Gram-negative bacteria, recovered from aerosols, to lysozyme and other hydrolytic enzymes. Journal of General Microbiology 61:197–204
    [Google Scholar]
  9. Henderson D. W. 1952; An apparatus for the study of airborne infection. Journal of Hygiene, Cambridge 50:53–68
    [Google Scholar]
  10. Heppel L. A. 1969; The effect of osmotic shock on release of bacterial proteins and on active transport. Journal of General Physiology 54:95–109
    [Google Scholar]
  11. Leive L. 1968; Studies on the permeability change produced in coliform bacteria by ethylenediaminetetraacetate. Journal of Biological Chemistry 243:2373–2380
    [Google Scholar]
  12. Leive L., Shovlin V. K., Mergenhagen S. E. 1968; Physical, chemical and immunological properties of lipopolysaccharide released from Escherichia coli by ethylenediaminetetraacetate. Journal of Biological Chemistry 243:6384–6391
    [Google Scholar]
  13. May K. R., Harper G. J. 1957; The efficiency of various liquid impinger samplers in bacterial aerosols. British Journal of Industrial Medicine 14:287–297
    [Google Scholar]
  14. Noller E. C., Hartsell S. E. 1961; Bacteriolysis of Enterobacteriaceae. 1. Lysis by four lytic systems utilizing lysozyme. Journal of Bacteriology 81:482–491
    [Google Scholar]
  15. Ouchterlony O. 1953; Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion. Actapathologica et microbiologica scandinavica 32:231–240
    [Google Scholar]
  16. Repaske R. 1958; Lysis of Gram-negative organisms and the role of versene. Biochimica et biophysica acta 30:225–232
    [Google Scholar]
  17. Rogers S. W., Gilleland H. E.Jun Eagon R. G. 1969; Characterization of a protein-lipopoly- saccharide complex released from cell walls of Pseudomonas aeruginosa by ethylenediaminetetraacetic acid. Canadian Journal of Microbiology 15:743–748
    [Google Scholar]
  18. Salton M. R. J. 1957; The properties of lysozyme and its action on micro-organisms. Bacteriological Reviews 21:82–99
    [Google Scholar]
  19. Voss J. G. 1967; Effects of organic cations on the Gram-negative cell wall and their bactericidal activity with EDTA and surface active agents. Journal of General Microbiology 48:391–400
    [Google Scholar]
  20. Webb S. J. 1969; The effect of oxygen on the possible repair of dehydration damage by Escherichia coli. Journal of General Microbiology 58:317–326
    [Google Scholar]
  21. Wilkinson S. G. 1968; Studies on the cell walls of Pseudomonas species resistant to EDTA. Journal of General Microbiology 54:195–213
    [Google Scholar]
  22. Winshell E. B., Neu H. C. 1970; Relation of lipopolysaccharide and fatty acid ester release to the ethylenediaminetetraacetic acid alteration of permeability in Enterobacteriaceae. Journal of Bacteriology 102:537–539
    [Google Scholar]
  23. Work E. 1967; Factors affecting the susceptibility of bacterial cell-walls to the action of lysozyme. Proceedings of the Royal Society B167:446–447
    [Google Scholar]
/content/journal/micro/10.1099/00221287-69-1-81
Loading
/content/journal/micro/10.1099/00221287-69-1-81
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error