1887

Abstract

SUMMARY: The walls of two mutants of and of the parent grown under the same conditions were identical in qualitative composition, with the possible exception of -5 grown on minimal medium without added sodium chloride; these walls did not contain galactosamine. The amounts of α, e-diamino-pimelic acid and glutamic acid in the walls of the mutants were lowered by growth on minimal media supplemented by o·8 -sodium chloride, whereas in the parent these growth conditions led to increases in the contents of these two amino acids. The mucopeptide of mutant -4 was very little cross-linked when growing under conditions that lead to a round form, but this also applied to the mucopeptide of the parent grown under the same conditions. When the mutant grew as a rod, its mucopeptide again had the same degree of cross-linking as that of the parent grown in rich media. The concentration of organic phosphorus in the walls of the mutants, presumed to be present as teichoic acid, was about the same as that in the parent and was unaffected by the growth conditions. Thus the chemistry of the walls of mutants did not provide obvious evidence of expression of the genetic lesion.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-66-3-297
1971-06-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/66/3/mic-66-3-297.html?itemId=/content/journal/micro/10.1099/00221287-66-3-297&mimeType=html&fmt=ahah

References

  1. Allen R. J. L. 1940; The estimation of phosphorus. Biochemical Journal 34:858–865
    [Google Scholar]
  2. Archibald A. R., Baddiley J. 1966; The teichoic acids. Advances in Carbohydrate Chemistry 21:323–375
    [Google Scholar]
  3. Baar S. 1954; Quantitative estimations of glucose by partition chromatography. Biochemical Journal 58:175–176
    [Google Scholar]
  4. Boylan R. J., Mendelson N. H. 1969; Initial characterization of a temperature-sensitive rod-mutant of Bacillus subtilis. Journal of Bacteriology 100:1316–1321
    [Google Scholar]
  5. Cole R. M., Popkin T. J., Boylan R. J., Mendelson N. H. 1970; Ultrastructure of a temperature- sensitive rod-mutant of Bacillus subtilis. Journal of Bacteriology 103:793–810
    [Google Scholar]
  6. Dreywood R. 1946; Qualitative test for carbohydrate material. Industrial and Engineering Chemistry (analytical edition) 18:499
    [Google Scholar]
  7. Ghuysen J. M. 1968; Use of bacteriolytic enzymes in determination of wall structure and their role in cell metabolism. Bacteriological Reviews 32:425–464
    [Google Scholar]
  8. Heptinstall S., Archibald A. R., Baddiley J. 1971; Teichoic acids and membrane function in bacteria. Nature, London 225:519–521
    [Google Scholar]
  9. Hughes R. C. 1968a; The cell wall of Bacillus licheniformis nctc 6346 Composition of the mucopeptide component. Biochemical Journal 106:41–48
    [Google Scholar]
  10. Hughes R. C. 1968b; The cell wall of Bacillus licheniformis nctc 6346 Isolation of low molecular weight fragments from the soluble mucopeptide. Biochemical Journal 106:49–59
    [Google Scholar]
  11. Hughes R. C. 1970; Autolysis of isolated cell walls of Bacillus licheniformis nctc 6346 and Bacillus subtilis Marburg strain 168. Biochemical Journal 119:849–860
    [Google Scholar]
  12. Hughes R. C., Pavlik J. G., Rogers H. J., Tanner P. J. 1968; Organization of polymers in the cell walls of some bacilli. Nature, London 219:642–644
    [Google Scholar]
  13. Hughes R. C., Tanner P. J. 1968; The action of dilute alkali on bacterial cell walls. Biochemical and Biophysical Research Communications 33:22–27
    [Google Scholar]
  14. Janczura E., Perkins H. R., Rogers H. J. 1960; Teichuronic acid: a mucopolysaccharide present in cell wall preparations from vegetative cells of Bacillus subtilis. Biochemical Journal 80:82–93
    [Google Scholar]
  15. Krulwich T. A., Ensign J. C., Tipper D. J., Strominger J. L. 1967a; Sphere-rod morphogenesis in Arthrobacter crystallopoietes I. Cell wall composition and polysaccharides of the peptidoglycan. Journal of Bacteriology 94:734–740
    [Google Scholar]
  16. Krulwich T. A., Ensign J. C., Tipper D. J., Strominger J. L. 1967b; Sphere-rod morphogenesis in Arthrobacter crystallopoietes II. Peptides of the cell wall peptidoglycan. Journal of Bacteriology 94:741–750
    [Google Scholar]
  17. Mandelstam J., Rogers H. J. 1959; The incorporation of amino acids into cell-wall mucopeptide of staphylococci and the effect of antibiotics on the process. Biochemical Journal 72:654–662
    [Google Scholar]
  18. Mirelman D., Sharon N. 1968; Isolation and characterization of the disaccharide N-acetylglucosaminyl- β-(1→ 4)-N-acetylmuramic acid and two tripeptide derivatives of this disaccharide from lysozyme digests of Bacillus licheniformis atcc 9945 cell walls. Journal of Biological Chemistry 243:2279–2287
    [Google Scholar]
  19. Partridge S. M. 1948; Filter-paper partition chromatography of sugars. Biochemical Journal 42:238–242
    [Google Scholar]
  20. Rogers H. J. 1969; The organization of polymers in Gram-positive and Gram-negative bacteria. Society for General Microbiology 57:iv
    [Google Scholar]
  21. Rogers H. J. 1970; Bacterial growth and the cell envelope. Bacteriological Reviews 34:194–214
    [Google Scholar]
  22. Rogers H. J., Perkins H. R. 1968 Cell Walls and Membranes London: E. & F. N. Spon;
    [Google Scholar]
  23. Rogers H. J., McConnell M., Burdett I. D. J. 1968; Cell-wall or membrane mutants of Bacillus subtilis and Bacillus licheniformis with grossly deformed morphology. Nature, London 219:285–288
    [Google Scholar]
  24. Rogers H. J., McConnell M., Burdett I. D. J. 1970; The isolation and characterization of mutants of Bacillus subtilis and Bacillus licheniformis with disturbed morphology and cell division. Society for General Microbiology 61:155–171
    [Google Scholar]
  25. Rogers H. J., McConnell M. 1970; The role of l-glutamine in the phenotypic change of a rod mutant derived from Bacillus subtilis 168. Society for General Microbiology 61:173–181
    [Google Scholar]
  26. Rosen H. 1957; A modified ninhydrin colorimetric analysis for amino acids. Archives of Biochemistry and Biophysics 67:10–15
    [Google Scholar]
  27. Schaechter M., Maaløe O., Kjeldegaard N. O. 1958; Dependency on medium and temperature of cell size, and chemical composition during balanced growth of Salmonella typhimurium. Society for General Microbiology 19:592–606
    [Google Scholar]
  28. Spizizen J. 1958; Transformation of biochemically deficient strains of Bacillus subtilis by DNA. Proceedings of the National Academy of Sciences of the United States of America 44:1072
    [Google Scholar]
  29. Warth A. D., Strominger J. L. 1969; Structure of the peptidoglycan of bacterial spores: occurrence of the lactam of muramic acid. Proceedings of the National Academy of Sciences of the United States of America 64:528–535
    [Google Scholar]
  30. Young F. E. 1966a; Fractionation and partial characterization of the products of autolysis of cell walls of Bacillus subtilis. Journal of Bacteriology 92:839–846
    [Google Scholar]
  31. Young F. E. 1966b; Autolytic enzyme associated with cell walls of Bacillus subtilis. Journal of Biological Chemistry 241:3462–3467
    [Google Scholar]
  32. Young F. E. 1967; Requirement of glucosylated teichoic acid for adsorption of phage in Bacillus subtilis 168. Proceedings of the National Academy of Sciences of the United States of America 58:2377–2384
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-66-3-297
Loading
/content/journal/micro/10.1099/00221287-66-3-297
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error