1887

Abstract

SUMMARY: is mutated by exposure to nitrosoguanidine at 0.1 and 1.0 mg./ml. for 15 and 60 min. After incubation for 24 h. in a complete medium, to allow for phenotypic expression, isoniazid (at 0.1 mg./ml. for 24 h.) is used to reduce the numbers of unwanted mutants and surviving wild-type bacteria in a procedure analagous to penicillin selection with other bacteria.

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/content/journal/micro/10.1099/00221287-66-1-115
1971-04-01
2021-10-26
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References

  1. Adelberg E. A., Mandel M., Chen G. C. C. 1965; Optimal conditions for mutagenesis by N-methyl-N’-nitro-N-nitrosoguanidine in Escherichia coli k12. Biochemical and Biophysical Research Communications 18:788–795
    [Google Scholar]
  2. Holland K. T., Ratledge C. 1970; Auxotrophic mutants of Mycobacterium smegmatis.. Society for General Microbiology 61:iii–iv
    [Google Scholar]
  3. Konicek J., Malek I. 1968; The application of the penicillin technique for the isolation of auxotrophic mutants. in Mycobacterium phlei strain pn. Folia microbiologica 13:158–160
    [Google Scholar]
  4. Konichova-Radochova M., Malek I. 1969; The mutagenic effect of nitrosoguanidine on Mycobacterium phlei pa.. Folia microbiologica 14:201–207
    [Google Scholar]
  5. Konichova-Radochova M., Konicek J., Malek I. 1970; The study of mutagenesis in Mycobacterium phlei.. Folia microbiologica 15:88–102
    [Google Scholar]
  6. Lederberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants. Journal of Bacteriology 63:399–406
    [Google Scholar]
  7. Ratledge C., Hall M. J. 1970; Uptake of salicyclic acid into mycobactin S by growing cells of Mycobacterium smegmatis. FEBS Letters 10:309–312
    [Google Scholar]
  8. Winder F. 1964; The antibacterial action of streptomycin, isoniazid and PAS.. In Chemotherapy of Tuberculosis pp 111–149 Edited by Barry V. C. London: Butterworths;
    [Google Scholar]
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