1887

Abstract

SUMMARY: The effect of increasing concentrations of glucose and galactose on the physiology and composition of was studied in continuous culture. Glucose, at concentrations ranging from 1 to 50 g./l. was degraded predominantly by aerobic fermentation. Galactose catabolism was mainly aerobic at concentrations up to 5 g./l. but became increasingly fermentative at higher sugar concentrations. The fatty-acid content varied with the type of metabolism. Thus at low concentrations of sugar, fatty acids accounted for 8 % dry wt but about 5 % dry wt at high sugar concentrations. High sugar concentrations may be associated with the repression of mitochondrial structures.

The size of the amino acid pool was a function of the availability of ammonia in the medium. Such ‘pools’ contained appreciable amounts of arginine when NH+ was in excess, whereas in the absence of NH+ from the culture filtrate only trace amounts of arginine were detected. The content of glutamic dehydrogenase in the yeast varied directly with the availability of ammonia and the size of the amino acid pool.

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/content/journal/micro/10.1099/00221287-64-3-279
1970-12-01
2022-01-18
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References

  1. Babij T., Moss F. J., Ralph B. J. 1969; Effects of oxygen and glucose levels on lipid composition of the yeast Candida utilis grown in continuous culture.. Biotechnology and Bioengineering 11:593
    [Google Scholar]
  2. Bartley W. 1964 Metabolism and Physiological Significance of Lipids378 Edited by Dawson R. M. C., Rhodes D. N. London: Wiley.;
    [Google Scholar]
  3. Brown C. M. 1970; The amino acid pools of yeasts.. Journal of General Microbiology 58:iii
    [Google Scholar]
  4. Brown C. M., Hough J. S. 1965; Elongation of yeast cells in continuous culture.. Nature, London 206:676
    [Google Scholar]
  5. Brown C. M., Rose A. H. 1969a; Effects of temperature on composition and cell volume of Candida utilis.. Journal of Bacteriology 97:261
    [Google Scholar]
  6. Brown C. M., Rose A. H. 1969b; Fatty acid composition of Candida utilis as affected by growth temperature and dissolved oxygen tension.. Journal of Bacteriology 99:371
    [Google Scholar]
  7. Criddle R. S., Schatz G. 1969; Promitochondria of anaerobically grown yeast. I. Isolation and biochemical properties.. Biochemistry 8:322
    [Google Scholar]
  8. Dawson P. S. S. 1965; The intracellular amino acid pool of Candida utilis during growth in batch and continuous flow culture.. Biochimica et biophysica acta 111:51
    [Google Scholar]
  9. De Deken R. H. 1966; The Crabtree effect, a regulatory system in yeast.. Journal of General Microbiology 44:149
    [Google Scholar]
  10. Fawcett J. K., Scott J. E. 1960; A rapid and precise method for the determination of urea.. Journal of Clinical Pathology 13:156
    [Google Scholar]
  11. Harris G., Parsons R. 1958; Nitrogenous constituents of brewing materials. XI. Nucleic acids of malt and their fate during brewing.. Journal of the Institute of Brewing 64:308
    [Google Scholar]
  12. Hommes F. A. 1966; Effect of glucose on the level of glycolytic enzymes in yeast.. Archives of Biochemistry and Biophysics 114:231
    [Google Scholar]
  13. Kates M. 1964; Simplified procedures for hydrolysis or methanolysis of lipids.. Journal of Lipid Research 5:132
    [Google Scholar]
  14. Lowry O. H., Rosebrough M. R., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265
    [Google Scholar]
  15. Marinetti G. V. 1962; Chromatographic separation, identification and analysis of phosphatides. Journal of Lipid Research 3:1
    [Google Scholar]
  16. Middlehoven W. J. 1970; Induction and repression of arginase and ornithine transaminase in baker’s yeast.. Antonie van Leeuwenhook 36:1
    [Google Scholar]
  17. Paltauf F., Schatz G. 1969; Promitochondria of anaerobically grown yeast. II. Lipid composition.. Biochemistry 8:335
    [Google Scholar]
  18. Polakis E. S., Bartley W., Meek G. A. 1965; Changes in the activities of respiratory enzymes during the aerobic growth of yeast on different carbon sources.. Biochemical Journal 97:298
    [Google Scholar]
  19. Slonimski P. P. 1956; Adaptation respiratoire: développement de la systèhemoproteique induit par l’oxygène.. International Congress of Biochemistry 3:242
    [Google Scholar]
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