1887

Abstract

SUMMARY

Inability of the L-phase of Gram-positive bacteria to persist is explainable by its need for osmotic protection. Adaptation to physiological osmotic conditions did not enhance the ability of the L-phase to survive after intravenous or intraperitoneal injection in mice. One ‘low-salt’ L-strain survived in mice for 8 days when protected against phagocytosis by a filter box, whereas under the same conditions its ‘high-salt’ parent L-strain died in a few hours. The decrease of the number of surviving colony-forming units of this low-salt L-strain in the filter box contrasts sharply with the increase in number shown by the parent bacterial strain protected in the same way.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-62-2-195
1970-08-01
2024-12-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/62/2/mic-62-2-195.html?itemId=/content/journal/micro/10.1099/00221287-62-2-195&mimeType=html&fmt=ahah

References

  1. Clasener H. A. L., Ensering H. L. 1970a; Search for the L-phase of Streptococcus faecalis in kidneys of rats experimentally infected with the bacterial phase and treated with penicillin. Annals of the New York Academy of Sciences In Press
    [Google Scholar]
  2. Clasener H. A. L., Ensering H. L. 1970b; The possible role of bacteriocins in the disappearance in vivo of the L-phase of group A haemolytic streptococci. Kohler M. Edited by In Proceedings of the Fourth International Symposium on Streptococcus pyogenes Jena: 29 September to 3 October 1969. (In preparation.)
    [Google Scholar]
  3. Freimer E. H., Krause R. M., McCarty M. 1959; Studies of L-forms and protoplasts of group A streptococci. Isolation, growth and bacteriological characteristics. Journal of Experimental Medicine no:853–874
    [Google Scholar]
  4. Guze L. B., Kalmanson G. M. 1964; Persistence of bacteria in ‘protoplast’ form after apparent cure of myelonephritis in rats. Science; New York: 1431340–1341
    [Google Scholar]
  5. Hijmans W., van Boven C. P. A., Clasener H. A. L. 1969; Fundamental biology of the L-phase of bacteria. In The Mycoplasmatales and the L-phase of Bacteria Hayflick L. Edited by New York: Appleton Century Crofts;
    [Google Scholar]
  6. Hill L. R. 1968; The determination of deoxyribonucleic acid base compositions and its application to bacterial taxonomy. In Identification Methods for Microbiologists Part B Gibbs B. M., Shapton D. A. Edited by London and New York: Academic Press;
    [Google Scholar]
  7. Kagan G. Y., Mikhailova V. S. 1963; Isolation of L-forms of streptococci from the blood of patients with rheumatism and endocarditis. Journal of Hygiene, Epidemiology, Microbiology and Immunology 7:327–343 In Russian
    [Google Scholar]
  8. Kaklamanis E., Thomas L., Stravropoulos K., Borman I., Boshwitz C. 1969; Mycoplasmacidal action of normal tissue extracts. Nature; London: 221860–862
    [Google Scholar]
  9. Kalmanson G. M., Hubert E. G., Montgomerie J. Z., Guze L. B. 1968; Serum bactericidal activity against protoplasts. In Microbial Protoplasts, Spheroplasts and L-forms Guze L. B. Edited by Baltimore: Williams and Wilkins;
    [Google Scholar]
  10. Lack C. H. 1968; Experimental models of rheumatoid arthritis. Medical Clinics of North America 52:667–672
    [Google Scholar]
  11. Lorincz A. L., Priestley J. O., Jacobs P. H. 1958; Evidence for a humoral mechanism which prevents growth of dermatophytes. Journal of Investigative Dermatology 31:15–17
    [Google Scholar]
  12. McCarty M. 1952; The lysis of group A hemolytic streptococci by extracellular enzymes of Streptomyces albus. I. Production and fractionation of the lytic enzymes. II. Nature of the cellular substrate attacked by the lytic enzymes. Journal of Experimental Medicine 96:555–568
    [Google Scholar]
  13. McCarty M. 1964; Missing links in the streptococcal chain leading to rheumatic fever. Circulation 29:488–493
    [Google Scholar]
  14. Minck R. 1954; Action des broyats d’organes sur les formes L du vibrion cholérique. Compte rendu des Séances de la Société de Biologie 148:715–717
    [Google Scholar]
  15. Muschel L. H. 1968; The formation of spheroplasts by immune substances and the reactivity of immune substances against diverse rounded forms. In Microbial Protoplasts, Spheroplasts and L-forms Guze L. B. Edited by Baltimore: Williams and Wilkins;
    [Google Scholar]
  16. Rickles N., Zilberstein Z., Kraus S., Arad G., Kaufstein M., Ginsburg I. 1969; Persistence of group A streptococci labelled with fluorescein isothiocyanate in inflammatory sites in the heart and muscle of mice and rabbits. Proceedings of the Society for Experimental Biology and Medicine 131:525–530
    [Google Scholar]
  17. Schmitt-Slomska J., Sacquet E., Caravano R. 1967; Group A streptococcal L-forms. I. Per-sistence among inoculated mice. Journal of Bacteriology 93:451–455
    [Google Scholar]
  18. Schmitt-Slomska J., Mauss H. 1968; Répartition des formes L du streptocoque du groupe A dans l’organisme de la souris après inoculation intrapéritonéale. Compte rendu hebdomadaire des Sciences, Paris 267:380–381
    [Google Scholar]
  19. Schmitt-Slomska J., Boue A., Caravano R. 1968; A carrier state in human diploid cell cultures infected with L-forms of group A streptococcus. In Current Research on Group A Streptococcus Caravano R. Edited by Amsterdam: Excerpta Medica Foundation;
    [Google Scholar]
  20. Skarnes R. C., Watson D. W. 1957; Antimicrobial factors of normal tissues and fluids. Bacteriological Reviews 21:273–294
    [Google Scholar]
  21. Tully J. G., Rask-Nielsen R. 1967; Mycoplasma in leukemic mice. Annals of the New York Academy of Sciences 143:345–352
    [Google Scholar]
  22. Watson R. F., Hirst G. K., Lancefield R. C. 1961; Bacteriological studies of cardiac tissues obtained at autopsy from eleven patients dying with rheumatic fever. Arthritis and Rheumatism 4:74–85
    [Google Scholar]
/content/journal/micro/10.1099/00221287-62-2-195
Loading
/content/journal/micro/10.1099/00221287-62-2-195
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error