@article{mbs:/content/journal/micro/10.1099/00221287-62-2-195, author = "Clasener, H. A. L. and Ensering, Henriette L. and HIJMANS, W.", title = "Persistence in Mice of the L-Phase of Three Streptococcal Strains Adapted to Physiological Osmotic Conditions", journal= "Microbiology", year = "1970", volume = "62", number = "2", pages = "195-202", doi = "https://doi.org/10.1099/00221287-62-2-195", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-62-2-195", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY Inability of the L-phase of Gram-positive bacteria to persist in vivo is explainable by its need for osmotic protection. Adaptation to physiological osmotic conditions did not enhance the ability of the L-phase to survive in vivo after intravenous or intraperitoneal injection in mice. One ‘low-salt’ L-strain survived in mice for 8 days in vivo when protected against phagocytosis by a filter box, whereas under the same conditions its ‘high-salt’ parent L-strain died in a few hours. The decrease of the number of surviving colony-forming units of this low-salt L-strain in the filter box contrasts sharply with the increase in number shown by the parent bacterial strain protected in the same way.", }