%0 Journal Article %A Clasener, H. A. L. %A Ensering, Henriette L. %A HIJMANS, W. %T Persistence in Mice of the L-Phase of Three Streptococcal Strains Adapted to Physiological Osmotic Conditions %D 1970 %J Microbiology, %V 62 %N 2 %P 195-202 %@ 1465-2080 %R https://doi.org/10.1099/00221287-62-2-195 %I Microbiology Society, %X SUMMARY Inability of the L-phase of Gram-positive bacteria to persist in vivo is explainable by its need for osmotic protection. Adaptation to physiological osmotic conditions did not enhance the ability of the L-phase to survive in vivo after intravenous or intraperitoneal injection in mice. One ‘low-salt’ L-strain survived in mice for 8 days in vivo when protected against phagocytosis by a filter box, whereas under the same conditions its ‘high-salt’ parent L-strain died in a few hours. The decrease of the number of surviving colony-forming units of this low-salt L-strain in the filter box contrasts sharply with the increase in number shown by the parent bacterial strain protected in the same way. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-62-2-195