1887

Abstract

SUMMARY

Mutants () have been isolated from one strain of and two of after treatment with -methyl-3-nitro--nitroso-guanidine, by replica plating from media containing 0·8 -NaCl to media of low salt content. When grown on the latter media these mutants appear as groups or strings of coccal bodies which, when examined in section under the electron microscope, show gross distortions in their walls and membranes; septum formation is greatly disorganized. When grown in media containing 0·8 to 1·0-NaCl, or KC1, or ample supplies of organic nitrogen, considerable correction of the morphology of one class of these mutants occurs. The other class of mutants is not changed to rods by growth in media of high salt content but is so changed by growth on rich media containing yeast extract. All the mutants revert to the parent type, but at very different rates. The physiological characteristics of the mutants and the parents are in most respects identical.

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/content/journal/micro/10.1099/00221287-61-2-155
1970-05-01
2021-10-16
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References

  1. Adler H. I., Terry C. E., Hardigree A. A. 1968; Giant cells of Escherichia coli . Journal of Bacteriology 95:139
    [Google Scholar]
  2. Baesel J., Douglas H. C. 1968; Osmotic remedial response in a galactose-negative mutant of Saccharomyces cerevisiae . Journal of Bacteriology 95:1103
    [Google Scholar]
  3. Boylan R. J., Mendelson N. H. 1969; Initial characterization of a temperature-sensitive rod mutant of Bacillus subtilis . Journal of Bacteriology 100:1316
    [Google Scholar]
  4. Cripps R. E., Work E. 1967; The accumulation of extracellular macromolecules by Staphylococcus aureus grown in the presence of sodium chloride and glucose. Journal of General Microbiology 49:127
    [Google Scholar]
  5. Gladstone G. P., Fildes P. 1940; A simple culture medium for general use without meat extract or peptone. British Journal of Experimental Pathology 21:161
    [Google Scholar]
  6. Hawthorne D. C., Fries J. 1964; Osmotic-remedial mutants. A new classification for nutri-tional mutants in yeast. Genetics, Princeton 50:829
    [Google Scholar]
  7. Hirota Y., Ryter A., Jacob F. 1968; Thermosensitive mutants of Escherichia coli affected in the process of DNA synthesis and cellular division. Cold Spring Harbor Symposia on Quantitative Biology 33:677
    [Google Scholar]
  8. Van Iterson W. 1961; Some features of a remarkable organelle in Bacillus subtilis . Journal of Biophysical and Biochemical Cytology 9:183
    [Google Scholar]
  9. Jacob F., Ryter A., Cuzin F. 1966; On the association between DNA and membrane in bacteria. Proceedings of the Royal Society Ser B 164:267
    [Google Scholar]
  10. Janczura E., Perkins H. R., Rogers H. J. 1960; Teichuronic acid: a mucopolysaccharide present in wall preparations from vegetative cells of Bacillus subtilis . Biochemical Journal 80:82
    [Google Scholar]
  11. Kellenberger E. 1960; The physical state of the bacterial nucleus. In Microbial Genetics 10:39 Symposia of the Society for General Microbiology
    [Google Scholar]
  12. Kuwana H. 1961; Studies on a temperature-sensitive irreparable mutant of Neurospora crassa. II. Osmotic nature of the mutant b 39 a. Japanese Journal of Genetics 36:187
    [Google Scholar]
  13. Landman O. E., Halle S. 1963; Enzymically and physically induced inheritance changes in Bacillus subtilis . Journal of Molecular Biology 7:721
    [Google Scholar]
  14. Landman O. E., Ryter A., Fréhel C. 1968; Gelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form. Electron-microscopic and physical study. Journal of Bacteriology 96:2154
    [Google Scholar]
  15. Mangiarotti G., Apirion D., Schlessinger D. 1966; Selection of sucrose-dependent Escherichia coli to obtain enveloped mutants and fragile cultures. Science; New York: 153892
    [Google Scholar]
  16. Mendelson N. H., Gross J. D. 1967; Characterisation of a temperature-sensitive mutant of Bacillus subtilis defective in deoxyribonucleic acid replication. Journal of Bacteriology 94:1603
    [Google Scholar]
  17. Metzenberg R. L. 1968; Repair of multiple defects of a regulatory mutant of Neurospora by high osmotic pressure and reversion. Archives of Biochemistry and Biophysics 125:532
    [Google Scholar]
  18. Mickle H. 1948; Tissue disintegrator. Journal of the Royal Microscopical Society 68:10
    [Google Scholar]
  19. Normark S. 1969; Mutation in Escherichia coli KI2 mediating sphere-like envelopes and changed tolerance to ultraviolet irradiation and some antibiotics. Journal of Bacteriology 98:1274
    [Google Scholar]
  20. Rogers H. J., Perkins H. R. 1968 Cell Walls and Membranes London: E. and F. N. Spon Ltd;
    [Google Scholar]
  21. Rogers H. J., McConnell M. 1970; The role of l-glutamine in the phenotypic change of a rod mutant derived from Bacillus subtilis 168. Journal of General Microbiology 61:173
    [Google Scholar]
  22. Rogers H. J., McConnell M., Burdett I. D. J. 1968; Cell wall or membrane mutants of Bacillus subtilis and Bacillus licheniformis with grossly deformed morphology. Nature; London: 219285
    [Google Scholar]
  23. Ryter A. 1968; Association of the nucleus and the membrane of bacteria: a morphological study. Bacteriological Reviews 32:39
    [Google Scholar]
  24. Ryter A., Kellenberger E. 1958; Ltude au microscope electronique de plasmas contenant de l’acide desoxyribonucleique. I. Les nucléoides des bacteries en croissance active. Zeitschrift für Naturforschung 13B:597
    [Google Scholar]
  25. Ryter A., Landman O. E. 1964; Electron microscope study of the relationship between mesosome loss and the stable L state (or protoplast state) in Bacillus subtilis . Journal of Bacteriology 88:457
    [Google Scholar]
  26. Stamp Lord. 1947; The preservation of bacteria by drying. Journal of General Microbiology 1:251
    [Google Scholar]
  27. Venable J. H., Coggeshall R. 1965; A simplified lead citrate stain for use in electron microscopy. Journal of Cell Biology 25:407
    [Google Scholar]
  28. Wright H. D. 1933; The importance of adequate reduction of peptone in the preparation of media for the pneumococcus and other organisms. Journal of Pathology and Bacteriology 37:257
    [Google Scholar]
  29. Young F. 1966; Autolytic enzyme associated with cell walls of Bacillus subtilis . Journal of Biological Chemistry 241:3462
    [Google Scholar]
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