1887

Abstract

SUMMARY

When grown in broth cultures containing sodium chloride and glucose, JHM produced protein, RNA and DNA in the culture fluid. Maximum yields were obtained with about 4 % (w/v) sodium chloride and 0·1 % (w/v) glucose. Accumulation of macromolecules began at the end of exponential growth and was accompanied by a decrease in culture turbidity. Electron microscopy revealed considerable cellular lysis, arising from rupture of newly formed septa. Before lysis occurred, the cocci exhibited many morphological abnormalities. After incubation for about 15 hr the culture showed evidence of fresh growth and the resulting cocci were characterized by abormally thick and uneven walls.

The results suggest that there was interference with the regulation of cell-wall synthesis, perhaps due to loss of mesosomes, which were never seen in the cytoplasm of organisms grown in sodium chloride glucose broth.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-49-1-127
1967-10-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/49/1/mic-49-1-127.html?itemId=/content/journal/micro/10.1099/00221287-49-1-127&mimeType=html&fmt=ahah

References

  1. Alexander M., Heppel L. A., Hurwitz J. 1961; The purification and properties of micrococcal nuclease. J. biol. Chem 236:3014
    [Google Scholar]
  2. Allsop J., Work E. 1963; Cell walls of Propionibacterium species : fractionation and composition. Biochem. J 87:512
    [Google Scholar]
  3. Anfinsen C. B., Taniuchi H., Heins J. N., Suriano J. R. 1965; Studies on an extracellular nuclease of Staphylococcus aureus . Science, N. Y 150:368
    [Google Scholar]
  4. Barkulis S. S., Smith C., Boltralik J. J., Heymann H. 1964; Structure of streptococcal cell walls. J. biol. Chem 239:4027
    [Google Scholar]
  5. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J 62:315
    [Google Scholar]
  6. Campbell J. N., Evans J. B., Perry J. J., Niven C. F. 1961; An extracellular material elaborated by Micrococcus sodonensis . J. Bact 82:828
    [Google Scholar]
  7. Catlin B. W. 1960; Transformation of Neisseria meningitidis by deoxyribonucleates from cells and from culture slime. J. Bact 79:579
    [Google Scholar]
  8. Catlin B. W., Cunningham L. S. 1958; Studies of extracellular and intracellular bacterial deoxyribonucleic acids. J. gen. Microbiol 19:522
    [Google Scholar]
  9. Cunningham L., Catlin B. W., Privat De Garilhe M. 1956; A deoxyribonuclease of Micrococcus pyogenes . J. Am. chem. Soc 78:4642
    [Google Scholar]
  10. Demain A. L., Burg R. W., Hendlin D. 1965; Excretion and degradation of ribonucleic acid by Bacillus subtilis . J. Bact 89:640
    [Google Scholar]
  11. Dirksen M. L., Dekker C. A. 1960; Micrococcal nuclease: consideration of its mode of action. Biochem. biophys. Res. Commun 2:147
    [Google Scholar]
  12. Fitz-James P. 1964; Fate of the mesosomes of Bacillus megaterium during protoplasting. J. Bact 87:1483
    [Google Scholar]
  13. Ghuysen J. M., Strominger J. L. 1963; Structure of the cell wall of Staphylococcus aureus strain Copenhagen. I. Preparation of fragments by enzymic hydrolysis. Biochemistry 2:1110
    [Google Scholar]
  14. von Hippel P. H., Felsenfeld G. 1964; Micrococcal nuclease as a probe of DNA conformation. Biochemistry 3:27
    [Google Scholar]
  15. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  16. Luft J. H. 1961; Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol 9:409
    [Google Scholar]
  17. Mitchell P., Moyle J. 1957; Autolytic release and osmotic properties of ‘protoplasts’ from Staphylococcus aureus . J. gen. Microbiol 16:184
    [Google Scholar]
  18. Morse M. L., Carter C. E. 1949; The synthesis of nucleic acids in cultures of Escherichia coli strains b and b/r . J. Bact 58:317
    [Google Scholar]
  19. Ottolenghi E., Hotchkiss R. D. 1962; Release of genetic transforming agent from pneumococcal cultures during growth and disintegration. J. exp. Med 116:491
    [Google Scholar]
  20. Reddi K. K. 1961; Action of micrococcal phosphodiesterase on polyribonucleotides termined by guanylic acid. Biochim. biophys. Acta 47:47
    [Google Scholar]
  21. Ryter A., Landman O. E. 1964; Electron microscope study of the relationship between mesosome loss and the stable L-state (or protoplast state) in Bacillus subtilis . J. Bact 88:457
    [Google Scholar]
  22. Schindler C. A., Schuhardt V. T. 1965; Purification and properties of lysostaphin—a lytic agent for Staphylococcus aureus . Biochim. biophys. Acta 97:242
    [Google Scholar]
  23. Shockman G. D. 1965; Unbalanced cell-wall synthesis: autolysis and cell-wall thickening. Bact. Rev 29:345
    [Google Scholar]
  24. Smithies W. R., Gibbons N. E. 1955; The deoxyribose nucleic acid slime layer of some halophilic bacteria. Can. J. Microbiol 1:614
    [Google Scholar]
  25. Weibull C. 1965; Plasmolysis in Bacillus megaterium . J. Bact 89:1151
    [Google Scholar]
  26. Weidel W., Pelzer H. 1964; Bagshaped macromolecules—a new outlook on bacterial cell walls. Adv. Enzymol 26:193
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-49-1-127
Loading
/content/journal/micro/10.1099/00221287-49-1-127
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error