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SUMMARY: The vitamin requirements of 63 strains and the amino acid requirements of 40 strains belonging to the genus Leuconostoc have been determined. The amino acid requirements separate the genus into dextran and non-dextran formers and in particular indicated that non-dextran formers should be excluded from Leuconostoc mesenteroides. The growth factor requirements support the division of the leuconostocs by other methods into six species: Leuconostoc mesenteroides, L. dextranicum, L. paramesenteroides, L. lactis, L. cremoris and L. oenos.
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