1887

Abstract

SUMMARY: Alcohols, amino acids, organic acids and sugars were tested as carbon sources for the growth of in the light and dark. Growth was only recorded with acetate in the light. A carbon balance sheet of C-acetate assimilation showed a greater incorporation of C into polysaccharide and less released as CO in the light, compared with the dark. The primary products of C-acetate assimilation into the soluble fraction of the organisms were identified; after 10 sec. 40% of the total C present in this fraction was in succinic acid, 15% in citric acid and 16% in malic acid. The percentage of total C in this fraction present in succinic and malic acids decreased consistently with time, while that in citric acid initially increased before decreasing. After 10 sec. the specific activity of succinic acid was more than twice that of citric acid. 4 x 10 -monofluoroacetate (MFA) effectively inhibited the incorporation of C-acetate into tricarboxylic acid cycle intermediates and related compounds, and markedly inhibited CO evolution. 10 - (3-4, dichlorophenyl)--dimethyl urea (DCMU) did not significantly inhibit C-metabolism. The key enzymes of the glyoxylate cycle, isocitrate lyase (E. C. 4.1.3.1) and malate synthetase (E. C. 4.1.3.2) were found to be present in , and did not disappear in the absence of acetate, but even so growth was not recorded on acetate in the dark.

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/content/journal/micro/10.1099/00221287-48-1-127
1967-07-01
2019-12-13
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