Radioactive incorporation techniques have been employed to investigate the effect of isonicotinic acid hydrazide (INH) on biosynthetic reactions in var. BCG. INH has no measurable effect on incorporation of P phosphate, S sulphate or C glycine into whole cells, although it inhibits by 50% the incorporation of C acetate. Incorporation of P into trichloracetic acid (TCA) insoluble components of supernatant solutions from centrifuged disrupted bacteria appears to be inhibited soon after the cells are exposed to INH. Similar experiments with S show a lag of 30–40 hr before incorporation is influenced by the drug. Chemical fractionation of organisms grown in the presence of P show that the major effect of INH is exerted on incorporation into the ribonucleic acid fraction.


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