1887

Abstract

SUMMARY

Radioactive incorporation techniques have been employed to investigate the effect of isonicotinic acid hydrazide (INH) on biosynthetic reactions in var. BCG. INH has no measurable effect on incorporation of P phosphate, S sulphate or C glycine into whole cells, although it inhibits by 50% the incorporation of C acetate. Incorporation of P into trichloracetic acid (TCA) insoluble components of supernatant solutions from centrifuged disrupted bacteria appears to be inhibited soon after the cells are exposed to INH. Similar experiments with S show a lag of 30–40 hr before incorporation is influenced by the drug. Chemical fractionation of organisms grown in the presence of P show that the major effect of INH is exerted on incorporation into the ribonucleic acid fraction.

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/content/journal/micro/10.1099/00221287-47-3-379
1967-06-01
2021-08-04
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References

  1. Aldridge C., Felton F. G., Muchmore H. G., Ramsay H. H. 1959; A new liquid synthetic medium for the cultivation of Mycobacterium species. Am. Rev. resp. Dis 80:267
    [Google Scholar]
  2. Barclay W. R., Ebert R. H., Koch-Weser D. 1953; Mode of action of isoniazid. Am. Rev. Tuberc 67:490
    [Google Scholar]
  3. Dorough G. D., Seaton D. L. 1954; A method for the extraction and assay of nucleic acid fragments in tissues. J. Am. chem. Soc 76:2873
    [Google Scholar]
  4. Dubos R. J., Davis B. D. 1946; Factors affecting the growth of tubercle bacilli in liquid media. J. exp. Med 83:409
    [Google Scholar]
  5. Fiske C., SubbaRow Y. 1925; The colorimetric determination of phosphorus. J. biol. Chem 66:375
    [Google Scholar]
  6. Fox H. H. 1951; Synthetic tuberculostats. Isonicotinaldehyde thiosemicarbazone and some related compounds Abstr. Xllth int. Congr. pure appl. Chem p 299
    [Google Scholar]
  7. Gangadharam P. R. J., Harold F. M., Schaefer W. B. 1963; Selective inhibition of nucleic acid synthesis in Mycobacterium tuberculosis by isoniazid. Nature, Lond 198:712
    [Google Scholar]
  8. Koch-Weser D., Barclay W. R., Ebert R. H. 1955; The influence of isoniazid and streptomycin on acid fastness, tétrazolium reduction, growth and survival of tubercle bacilli. Am. Rev. Tuberc 71:556
    [Google Scholar]
  9. Krüger-Thiemer E. 1958; Isonicotinic acid hypothesis of the antituberculous action of isoniazid. Am. Rev. Tuberc 77:364
    [Google Scholar]
  10. Mackaness G. B., Smith N. 1953; The bactericidal action of isoniazid, streptomycin and terra-mycin on extra cellular and intra cellular tubercle bacilli. Am. Rev. Tuberc 67:322
    [Google Scholar]
  11. Meadow P. 1956; The action of isonicotinic acid hydrazide on the metabolism of Mycobacterium smegmatis. J. gen. Microbiol 14:406
    [Google Scholar]
  12. Meadow P., Knox R. 1956; The effect of isonicotinic acid hydrazide on the oxidative metabolism of Mycobacterium tuberculosis var. bovis bcg. J. gen. Microbiol 14:414
    [Google Scholar]
  13. Middlebrook G. 1952; Sterilization of tubercle bacilli by isonicotinic acid hydrazide and the incidence of variants resistant to the drug in vitro. Am. Rev. Tuberc 65:765
    [Google Scholar]
  14. Milner H. W., Lawrence N. S., French C. S. 1950; Colloidal dispersion of chloroplast material. Science N. Y 111:633
    [Google Scholar]
  15. Roberts R. B., Abelson P. H., Cowie D. B., Bolton E. T., Britten R. J. 1955; Studies of biosynthesis in Escherichia coli. Pubis Carnegie Instn p 607
    [Google Scholar]
  16. Schaefer W. B. 1954; The effect of isoniazid on growing and resting tubercle bacilli. Am. Rev. Tuberc 69:125
    [Google Scholar]
  17. Schmidt G., Thannhauser S. J. 1945; A method for the determination of desoxyribonucleic acid, ribonucleic acid and phosphoproteins in animal tissue. J. biol. Chem 161:83
    [Google Scholar]
  18. Strickland K. P., Thompson R. H. S., Webster G. R. 1956; The action of phosphatases of human brain on lipid phosphate esters. J. Neurol. Neurosurg. Psychiat 19:12
    [Google Scholar]
  19. Tsukamura M., Mizuno M. 1962; Mode of action of isoniazid viewed from isotope incorporation studies. Kekkaku 37:29
    [Google Scholar]
  20. Winder F. C., Denneny J. M. 1957; The metabolism of inorganic polyphosphate in Myco-bacteria. J. gen. Microbiol 17:573
    [Google Scholar]
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