1887

Abstract

SUMMARY: Some chemical requirements for stimulating the germination of spores of a few species of the genus were studied by means of viable counts.

Of the three compounds alanine, tyrosine and adenosine, -alanine was present in all cases where highly significant stimulation was observed; the need for the other two compounds ranged from ability to dispense completely with both of them to a distinct need for the two together to produce a maximum effect.

Generally, the effects were greater with spores grown in a chemically defined amino-acid medium aerated by shaking than with those grown on the surface of a casein-hydrolysate agar.

In the single case examined, that of a laboratory strain of , the effect of -alanine was strongly inhibited -alanine at a molar ratio of 1:30.

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1950-01-01
2024-12-13
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References

  1. Brewer C. R., Mccullough W. G., Mills R. C., Roessler W. G., Herbst E. J., Hows A. F. 1946; Studies on the nutritional requirements of Bacillus anthracis. Arch. Biochem 10:65
    [Google Scholar]
  2. Evans F. R., Curran H. R. 1943; The accelerating effect of sub-lethal heat on spore germination in mesophilic aerobic bacteria. J. Bact 46:513
    [Google Scholar]
  3. Fildes P. 1938; The growth of Proteus on ammonium lactate plus nicotinic acid. Brit. J. exp. Path 19:239
    [Google Scholar]
  4. Fisher R. A. 1946 Statistical methods for research workers, 10th ed. Edinburgh: Oliver and Boyd;
    [Google Scholar]
  5. Fling M., Fox S. W. 1945; Antipodal specificity in the inhibition of Lactobacillus arabinosus by amino-acids. J. biol. Chem 160:329
    [Google Scholar]
  6. Fox S. W., Fling M., Bollenbach G. N. 1944; Inhibition of bacterial growth by d-leucine. J. biol. Chem 155:465
    [Google Scholar]
  7. Gladstone G. P. 1939; Interrelationships between amino-acids in the nutrition of B. anthracis. Brit. J. exp. Path 20:189
    [Google Scholar]
  8. Gladstone G. P., Fildes P. 1940; A simple culture medium for general use without meat extract or peptone. Brit. J. exp. Path 21:161
    [Google Scholar]
  9. Hagan W. A., O’Kane D. J., Young G. A. 1943; Private communication.
    [Google Scholar]
  10. Hills G. M. 1949; Chemical factors in the germination of spore-bearing aerobes. The effect of yeast extract on the germination of Bacillus anthracis and its replacement by adenosine. Biochem. J 45:353
    [Google Scholar]
  11. Hills G. M. 1949; Chemical factors in the germination of spore-bearing aerobes. The effect of amino-acids in the germination of Bacillus anthracis including observations on the specificity of some stereoisomers. Biochem. J 45:363
    [Google Scholar]
  12. Keilin D., Haktree F. 1947; Comparative study of the spores and vegetative forms of Bacillus subtilis. Antonie van Leeuwenhoek J. Microbiol. Serol 12:115
    [Google Scholar]
  13. Knaysi G. 1945; Investigation of the existence and nature of reserve material in the endospore of a strain of Bacillus mycoides by an indirect method. J. Bact 49:617
    [Google Scholar]
  14. Knaysi G., Baker R. F. 1947; Demonstration, with the electron microscope, of a nucleus in Bacillus mycoides grown in a nitrogen-free medium. J. Bact 53: 539
    [Google Scholar]
  15. Kobayashi Y., Fling M., Fox S. W. 1948; Antipodal specificity in the inhibition of growth of Escherichia coli by amino-acids. J. biol. Chem 174:391
    [Google Scholar]
  16. Lamanna C. 1940; The taxonomy of the genus Bacillus. 1. Modes of spore germination. J. Bact 40: 347
    [Google Scholar]
  17. Miles A. A., Misra S. S. 1938; The estimation of the bactericidal power of blood. J. Hyg., Camb 38:732
    [Google Scholar]
  18. Robbins W. J., Kavanagh V. W., Kavanagh F. 1942; Growth substances and dormancy of spores of Phycomyces. Bot. Gaz 104:224
    [Google Scholar]
  19. Robinow C. F. 1942; A study of the nuclear apparatus of bacteria. Proc. Roy. Soc 130:299
    [Google Scholar]
  20. Work T. S. 1948; d- and l-amino-acids in antibiotics. Biochem. Soc. Symp 1:61
    [Google Scholar]
  21. Wynne E. S., Foster J. W. 1948; Physiological studies in spore germination with special reference to Clostridium botulinum. 1. Development of a quantitative method. J. Bact 55:61
    [Google Scholar]
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