SUMMARY: Some chemical requirements for stimulating the germination of spores of a few species of the genus were studied by means of viable counts.

Of the three compounds alanine, tyrosine and adenosine, -alanine was present in all cases where highly significant stimulation was observed; the need for the other two compounds ranged from ability to dispense completely with both of them to a distinct need for the two together to produce a maximum effect.

Generally, the effects were greater with spores grown in a chemically defined amino-acid medium aerated by shaking than with those grown on the surface of a casein-hydrolysate agar.

In the single case examined, that of a laboratory strain of , the effect of -alanine was strongly inhibited -alanine at a molar ratio of 1:30.


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