%0 Journal Article %A Hills, G. M. %T Chemical Factors in the Germination of Spore-bearing Aerobes: Observations on the Influence of Species, Strain and Conditions of Growth %D 1950 %J Microbiology, %V 4 %N 1 %P 38-47 %@ 1465-2080 %R https://doi.org/10.1099/00221287-4-1-38 %I Microbiology Society, %X SUMMARY: Some chemical requirements for stimulating the germination of spores of a few species of the genus Bacillus were studied by means of viable counts. Of the three compounds alanine, tyrosine and adenosine, l-alanine was present in all cases where highly significant stimulation was observed; the need for the other two compounds ranged from ability to dispense completely with both of them to a distinct need for the two together to produce a maximum effect. Generally, the effects were greater with spores grown in a chemically defined amino-acid medium aerated by shaking than with those grown on the surface of a casein-hydrolysate agar. In the single case examined, that of a laboratory strain of B. subtilis, the effect of l-alanine was strongly inhibited d-alanine at a molar ratio of 1:30. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-4-1-38