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It was shown that bilateral transfer of two episomes col I and RTF, carried singly in different strains of Shigella flexneri, could occur at the same time. The transfer of col I occurred at a considerably higher frequeney than did the transfer of RTF. Whilst the presence of col I in the acceptor organism appeared not to affect the frequency of transfer of RTF it was observed that, in some cases at least, the presence of RTF in the acceptor organism lowered the frequency of col I transfer.
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