1887

Abstract

SUMMARY

Partially purified preparations of the Kofi Pare isolate of cocoa swollen-shoot virus (CSSV) were usually inactivated after 10 min. at 50°, but not after 10 min. at 45°. Infectivity of freshly made preparations was greatly lessened by diluting 1/10, and lost at 1/100. The infectivity of preparations increased after storage for 24 hr; after 96 hr infectivity was retained at 0–4° but soon lost at 25°. The virus survived freezing and, with some loss of infectivity, freezing in leaves and storage in leaves dried over aluminium oxide. Infective material was precipitated from dilute extracts by half saturation with ammonium sulphate at 25°; it appeared to be equally stable over the range pH 6 to pH 8. Preparations of the sympto-matologically distinct Kofi Pare, Mampong, Dawa, Nsaba and Bosomuoso isolates all contained similar rod-shaped particles of size about 121 × 28 m.

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/content/journal/micro/10.1099/00221287-36-2-303
1964-08-01
2022-01-26
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References

  1. Baker R. E. D., Dale W. T. 1947; Notes on a virus disease of cacao. Ann. appl. Biol 34:60
    [Google Scholar]
  2. Brandes J., Paul H. L. 1957; Das Elektronenmikroskop als Hilfsmittel bei der Diagnose pflanzliche Virosen. Arch. Mikrobiol 26:358
    [Google Scholar]
  3. Brenner S., Horne R. W. 1959; A negative staining method for high resolution electron microscopy of viruses. Biochim. biophys. Acta 34:103
    [Google Scholar]
  4. Brunt A. A., Kenten R. H. 1960; Mechanical transmission of cocoa swollen-shoot virus. Virology 12:328
    [Google Scholar]
  5. Brunt A. A., Kenten R. H. 1962a; Virus research. Mechanical transmission of cocoa viruses. Rep. W. Afr. Cocoa Res. Inst 1960-61 p. 22
    [Google Scholar]
  6. Brunt A. A., Kenten R. H. 1962b; Mechanical transmission of cocoa swollen-shoot virus to and from cocoa and other hosts. Ann. appl. Biol 50:749
    [Google Scholar]
  7. Brunt A. A., Kenten R. H. 1963; The use of protein in the extraction of cocoa swollen-shoot virus from cocoa leaves. Virology 19:388
    [Google Scholar]
  8. Cadman C. H. 1959; Some properties of an inhibitor of virus infection from leaves of raspberry. J. gen. Microbiol 20:113
    [Google Scholar]
  9. Gibbs A. J., Nixon H. L., Woods R. D. 1963; Properties of purified preparations of lucerne mosaic virus. Virology 19:441
    [Google Scholar]
  10. Holden M. 1957; An investigation on polyphenolic compounds of the cocoa leaf in connexion with a chemical method for detecting virus infection. J. Sci. Fd Agric 8:553
    [Google Scholar]
  11. Posnette A. F. 1947; Virus diseases of cacao in West Africa. I. Cacao viruses 1A, 1B, lc and ID. Ann. appl. Biol 34:388
    [Google Scholar]
  12. Thresh J. M. 1956; Some effects of tannic acid and of leaf extracts which contain tannins on the infectivity of tobacco mosaic and tobacco necrosis viruses. Ann. appl. Biol 44:608
    [Google Scholar]
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