1887

Abstract

Summary: The best labelling of (var. ) with P was achieved when the organisms were grown in broth containing 33 g. ortho- phosphate/ml. and 150–200 c./ml. P. Cocci labelled in this way were as virulent to mice as control cocci. The phosphorus content of cocci was proportional to the orthophosphate content of the medium over the range 5 g.-300 g. PO/ml. P was released from cocci when they were incubated with phosphate buffer and other media which did not support cell division. P was released from labelled dividing cocci grown in nonradioactive media. About 80% of the P was released during division; a maximum of about 55% was released from non-dividing cocci over a similar time period. Most of the P released from dividing cocci was present in the form of molecules non-diffusible in dialysis (80%); most of the P released into phosphate buffer was diffusible. Cocci deficient or rich in phosphorus were fractionated with trichloroacetic acid (TCA), ether, ethanol and perchloric acid. Cocci containing low amounts of P lacked P in the fraction soluble in cold TCA, about 80% of which was orthophosphate. Cocci deficient in phosphorus incorporated P from POsolutions more rapidly than enriched cocci. The distributions of P in cocci previously rich or deficient in P were similar, but labelling in deficient cocci was increased in the RNA-containing fraction; and that part of the fraction soluble in hot TCA which had a barium salt insoluble at pH 4. The phosphorus level in the cells had no effect on the amount of phosphorus released into phosphate buffer solution.

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/content/journal/micro/10.1099/00221287-36-1-37
1964-07-01
2022-01-25
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