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SUMMARY: Seven actidione-resistant mutants recovered from separate strains of Neurospora crassa are due to mutations of single genes at either of two loci: act-1 (located between nit-1 and aur in linkage group I) and act-2 (located close to sp in linkage group V). Mutant alleles at both loci are dominant over their wild-type alleles in heterokaryons. Both genes are located in well marked chromosome arms, and scoring is distinct.
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