SUMMARY: A non-precipitating chemically defined medium containing lactate, sulphate and other inorganic salts supported repeated subculture of , strain Hildenborough; yields of bacteria were comparable with those obtained in media containing yeast extract or peptone. Addition of yeast extract, amino acid mixtures or ATP to the defined medium increased the crop. Growth on other organic substances was poorer than on lactate; amino acids were less efficient nitrogen sources than ammonia. Pyruvate served as electron acceptor for hydrogen uptake by resting organisms but did not support growth in sulphate-free medium.


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