1887

Abstract

SUMMARY:

Forward mutations at the locus in Neurospora, and back mutations at , were scored by using the ‘suppressor method’. This method is efficient and well suited for mutagen screening. Nitrous acid, ethyl-methane sulphonate and ultraviolet radiation were effective as mutagens; 8-ethoxycaffeine yielded inconclusive results. The same pattern of forward mutations at , but different frequencies of back mutations at the locus, were obtained with the three agents. In this Neurospora system, nitrous acid appeared to induce mutations which affected simultaneously both DNA strands.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-30-2-317
1963-02-01
2024-10-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/30/2/mic-30-2-317.html?itemId=/content/journal/micro/10.1099/00221287-30-2-317&mimeType=html&fmt=ahah

References

  1. Atwood K. C., Norman A. 1949; On the interpretation of multi-hit survival curves. Proc. nat. Acad. Sci., Wash 35:696
    [Google Scholar]
  2. Barratt R. W., Garnjobst L. 1949; Genetics of a colonial microconidiating mutant strain of Neurospora crassa. Genetics 34:351
    [Google Scholar]
  3. Beadle G. W., Tatum E. L. 1945; Neurospora. II. Methods of producing and detecting mutations concerned with nutritional requirements. Amer. J. Bot 32:678
    [Google Scholar]
  4. Geiduschek E. P. 1961; ‘Reversible’ DNA. Proc. nat. Acad. Sci., Wash 47:950
    [Google Scholar]
  5. Giles N. H. 1951; Studies on the mechanism of reversion in biochemical mutants of Neurospora crassa. Cold Spr. Harb. Symp. quant. Biol 16:283
    [Google Scholar]
  6. Grigg G. W. 1952; Back mutation assay method in microorganisms. Nature, Lond 169:98
    [Google Scholar]
  7. Kølmark G., Westergaard M. 1952; Validity of the Neurospora back-mutation test. Nature, Lond 169:626
    [Google Scholar]
  8. Lea D. E. 1947 Actions of Radiations on Living Cells Cambridge University Press;
    [Google Scholar]
  9. Mitchell M. B., Mitchell H. K. 1954; A partial map of linkage group D in Neurospora crassa. Proc. not. Acad. Sci., Wash 40:436
    [Google Scholar]
  10. Reissig J. L. 1958; A suppressor of citrulline dependence in Neurospora: a case of single-gene heterosis. Heredity 12:397
    [Google Scholar]
  11. Reissig J. L. 1959; Forward and back mutation at the pyr 3 locus of Neurospora. Heredity 13:144
    [Google Scholar]
  12. Reissig J. L. 1960; Forward and back mutation in the pyr 3 region of Neurospora. I. Mutations from arginine dependence to prototrophy. Genet. Res., Camb 1:356
    [Google Scholar]
  13. Reissig J. L. 1963; Spectrum of forward mutants in the pyr-B region of Neurospora. J. gen. Microbiol 30:327
    [Google Scholar]
  14. Sneed M. C., Brasted R. C. 1956 Comprehensive Inorganic Chemistry 573 Princeton: D. van Nostrand Co., Inc;
    [Google Scholar]
  15. Srb A. M., Horowitz N. H. 1944; The ornithine cycle in Neurospora and its genetic control. J. biol. Chem 154:129
    [Google Scholar]
  16. Teorell T., Stenhagen E. 1938; Ein Universalpuffer für den pH-Bereich 2, 0·12,0. Biochem. Z 299:416
    [Google Scholar]
  17. Tessman I. 1959; Mutagenesis in phages X174 and T4 and properties of the genetic material. Virology 9:375
    [Google Scholar]
  18. Vielmetter W., Wieder C. M. 1959; Mutagene und inaktivierende Wirkung salpetriger Säure auf freie Partikel des Phagen T2. Z. Naturforsch, leb312
    [Google Scholar]
  19. Westergaard M. 1960 Chemical mutagenesis as a tool in macromolecular genetics. In Chemische Mutagenese, Erwin-Baur Gedächtnisvorlesungen i, 1959 Abh. dtsch. Akad. Wiss., Berl Klasse für Medizin;1
    [Google Scholar]
  20. Westergaard M., Mitchell H. K. 1947; Neurospora V. A synthetic medium favoring sexual reproduction. Amer. J. Bot 34:573
    [Google Scholar]
  21. Witkin E. M. 1959; Post-irradiation metabolism and the timing of ultraviolet-induced mutations in bacteria. Proc. 10th int. Congr. Genet. 1:280
    [Google Scholar]
  22. Zetterberg G. 1960; The mutagenic effect of 8-ethoxycaffeine, caffeine and dimethyl-sulphate in the Ophiostoma back-mutation test. Hereditas 46:279
    [Google Scholar]
/content/journal/micro/10.1099/00221287-30-2-317
Loading
/content/journal/micro/10.1099/00221287-30-2-317
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error