1887

Abstract

SUMMARY:

A study of the nutritional requirements of has led to the development of a growth medium containing inorganic salts, amino acids, glucose, nucleosides, vitamins and 1% (w/v) bovine serum albumin fraction V. No cholesterol was detected in this medium. Potassium, magnesium and phosphate ions were essential growth requirements. Sodium chloride provided the high tonicity required by the growth medium. The addition of an ammonium salt to the medium enabled the growth of the test organism in the presence of 13 amino acids. Resting organisms of did not catabolize any of the 22 different amino acids tested under conditions which allowed amino acid degradation by . Glucose was essential for growth and could be replaced by maltose but not galactose, fructose, mannose, lactose or sucrose. The requirement for nucleic acid precursors was satisfied by adenosine, guanosine and cytidine, and that for vitamins by nicotinic acid, riboflavin, folinic acid, pyridoxine, pyridoxal and thiamine. Cholesterol was not necessary for growth of the test organism.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-30-1-141
1963-01-01
2022-11-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/30/1/mic-30-1-141.html?itemId=/content/journal/micro/10.1099/00221287-30-1-141&mimeType=html&fmt=ahah

References

  1. Adler H. E., Shifrine M. 1960; Nutrition, metabolism, and pathogenicity of mycoplasmas. Annu. Rev. Microbiol. 14:141
    [Google Scholar]
  2. Block R. J., Durrxjm E. L., Zweig G. 1958 A Manual of Paper Chromatography and Paper Electrophoresis, 2nd ed. New York: Academic Press Inc;
    [Google Scholar]
  3. Butler M., Knight B. C. J. G. 1960; The survival of washed suspensions of Mycoplasma. J. gen. Microbiol. 22:470
    [Google Scholar]
  4. Cook R. P. 1958 Cholesterol. New York: Academic Press Inc;
    [Google Scholar]
  5. Davis B. D., Dubos R. J. 1947; The binding of fatty acids by serum albumin, a protective growth factor in bacteriological media. J. exp. Med. 86:215
    [Google Scholar]
  6. Eagle H., Piez K. A. 1960; The utilization of proteins by cultured human cells. J. biol. Chem. 235:1095
    [Google Scholar]
  7. Edward D. G. ff. 1954; The pleuropneumonia group of organisms: a review, together with some new observations. J. gen. Microbiol. 10:27
    [Google Scholar]
  8. Edward D. G. ff., Fitzgerald W. A. 1951; Cholesterol in the growth of the pleuropneumonia group. J. gen. Microbiol. 5:576
    [Google Scholar]
  9. Edward D. G. ff., Freundt E. A. 1956; The classification and nomenclature of organisms of the pleuropneumonia group. J. gen. Microbiol. 14:197
    [Google Scholar]
  10. Freundt E. A. 1958 The Mycoplasmataceae (The pleuropneumonia group of organisms) Morphology, Biology and Taxonomy. Copenhagen: Munksgaard;
  11. Gilby A. R., Few A. V., McQuillen K. 1958; The chemical composition of the protoplast membrane of Micrococcus lysodeikticus. Biochim. biophys. Acta 29:21
    [Google Scholar]
  12. Giri K. V., Radhakrishnan A. N., Vaidyanathan C. S. 1952; A simple paper chromatographic method for the study of transamination reactions. Nature, Lond. 170:1025
    [Google Scholar]
  13. Hughes D. E. 1951; A press for disrupting bacteria and other micro-organisms. Brit. J. exp. Path. 32:97
    [Google Scholar]
  14. Kandler G., Handler O. 1955; Emährungs-und stoffwechselphysiologische Untersuchungen an pleuropneumonieähnlichen Organismen und der L-Phase der Bacteriën. ZU. Bakt. Abt. II 108:383
    [Google Scholar]
  15. Leach R. H. 1962; The osmotic requirements for growth of Mycoplasma. J. gen. Microbiol. 27:345
    [Google Scholar]
  16. Ma T. S., Zuazaga G. 1942; Microkjeldahl determination of nitrogen. Industr. Engng Chem. (Anal.) 14:280
    [Google Scholar]
  17. Mager J. 1959; Spermine as a protective agent against osmotic lysis. Nature, Lond. 183:1827
    [Google Scholar]
  18. Merrifield R. B., Dunn M. S. 1950; The microbiological determination of pyrrolidines with lactobacilli. J. biol. Chem. 186:331
    [Google Scholar]
  19. Neimark H. C., Pickett M. J. 1960; Products of glucose metabolism by pleuro-pneumonialike organisms. Ann. N.Y. Acad. Sci. 79:531
    [Google Scholar]
  20. Piez K. A., Oyama V. I., Levintow L., Eagle H. 1960; Proteolysis in stored serum and its possible significance in cell culture. Nature, Lond. 188:59
    [Google Scholar]
  21. Razin S. 1962a Nutrition and metabolism of pleuropneumonia-like organisms. Pleuropneumonia-like Organisms (PPLO) Mycoplasmataceae. Klieneberger-Nobel E. London: Academic Press Inc;
    [Google Scholar]
  22. Razin S. 1962b; Nucleic acid precursor requirements of Mycoplasma laidlawii. J. gen. Microbiol. 28:243
    [Google Scholar]
  23. Razin S., Argaman M. 1968; Lysis of Mycoplasma, bacterial protoplasts, spheroplasts and L-forms by various agents. J. gen. Microbial. 30:155
    [Google Scholar]
  24. Razin S., Knight B. C. J. G. 1960a; A partially defined medium for the growth of Mycoplasma. J. gen. Microbiol. 22:492
    [Google Scholar]
  25. Razin S., Knight B. C. J. G. 1960b; The effects of ribonucleic acid and deoxyribonucleic acid on the growth of Mycoplasma. J. gen. Microbiol. 22:504
    [Google Scholar]
  26. Razin S., Oliver O. 1961; Morphogenesis of Mycoplasma and Bacterial L-form colonies. J. gen. Microbiol. 24:225
    [Google Scholar]
  27. Rodwell A. W. 1956; The role of serum in the nutrition of Asterococcus mycoides. Aust. J. biol. Sci. 9:105
    [Google Scholar]
  28. Rodwell A. W. 1960; Nutrition and metabolism of Mycoplasma mycoides var. mycoides. Ann. N.Y. Acad. Sci. 79:499
    [Google Scholar]
  29. Rodwell A. W., Abbot A. 1961; The function of glycerol, cholesterol and long-chain fatty acids in the nutrition of Mycoplasma mycoides. J. gen. Microbiol. 25:201
    [Google Scholar]
  30. Rothblat G. H., Smith P. F. 1961; Nonsaponifiable lipids of representative pleuropneumonia-like organisms. J. Bact. 82:479
    [Google Scholar]
  31. Rozansky R., Bachrach U., Grossowicz N. 1954; Studies on the antibacterial action of spermine. J. gen. Microbiol. 10:11
    [Google Scholar]
  32. Salzman N. P. 1961; Animal cell cultures. Science 133:1559
    [Google Scholar]
  33. Smith P. F. 1955; Amino acid metabolism by pleuropneumonia-like organisms. I. General catabolism. J. Bact. 70:552
    [Google Scholar]
  34. Smith P. F. 1960; Amino acid metabolism of PPLO. Ann. N.Y. Acad. Sci. 79:543
    [Google Scholar]
  35. Smith P. F., Lynn R. J. 1958; Lipid requirements for the growth of pleuropneumonia-like organisms. J. Bact. 76:264
    [Google Scholar]
  36. Smith P. F., Morton H. E. 1951; The separation and characterization of the growth factor in serum and ascitic fluid which is required by certain pleuropneumonia-like organisms. J. Bact. 61:395
    [Google Scholar]
  37. Snell E. E. 1951 Bacterial nutrition-chemical factors in Bacterial Physiology. 214 Werkman C. H., Wilson P. W. New York: Academic Press Inc;
    [Google Scholar]
  38. Tabor H., Tabor C. W., Rosenthal S. M. 1961; The biochemistry of the polyamines, spermidine and spermine. Ann. Rev. Biochem. 30:579
    [Google Scholar]
  39. Tourtellotte M. E., Jacobs R. E. 1960; Physiological and serological comparisons of PPLO from various sources. Ann. N.Y. Acad. Sci. 79:521
    [Google Scholar]
  40. Umbreit W. W., Burris R. H., Stauffer J. F. 1957 Manometric Techniques. Minneapolis: Burgess Publishing Co;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-30-1-141
Loading
/content/journal/micro/10.1099/00221287-30-1-141
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error