SUMMARY: Murray Valley encephalitis virus was grown in baby mouse brain and purified. In five out of six experiments, the number of virus particles per chick egg LD50 was 90 ± 50. Fifteen preparations of purified virus were pooled and the pool examined chemically. The nucleic acid content was 7·8%, with bases present in the proportions: adenylic acid, 25·5; guanylic acid, 27·5; cytidylic acid, 21·5; uridylic acid, 25·5. No thymidylic acid was found. The virus preparation contained 11% lipid; phospholipid and total cholestorol were present to 0·8% and 1·6%, respectively, of the virus preparation.


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