1887

Abstract

SUMMARY: Murray Valley encephalitis virus was grown in baby mouse brain and purified. In five out of six experiments, the number of virus particles per chick egg LD50 was 90 ± 50. Fifteen preparations of purified virus were pooled and the pool examined chemically. The nucleic acid content was 7·8%, with bases present in the proportions: adenylic acid, 25·5; guanylic acid, 27·5; cytidylic acid, 21·5; uridylic acid, 25·5. No thymidylic acid was found. The virus preparation contained 11% lipid; phospholipid and total cholestorol were present to 0·8% and 1·6%, respectively, of the virus preparation.

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/content/journal/micro/10.1099/00221287-29-1-165
1962-09-01
2024-12-06
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References

  1. Ada G. L., Anderson S. G., Abbot A. 1961; Purification of Murray Valley encephalitis virus. J. gen. Microbiol. 24:177
    [Google Scholar]
  2. Ada G. L., Perry B. T. 1956; Influenza virus nucleic acid: relationship between biological characteristics of the virus particle and properties of the nucleic acid. J. gen. Microbiol. 14:623
    [Google Scholar]
  3. Anderson S. G., Ada G. L. 1959; Murray Valley encephalitis virus: preparation of an infective ‘ribonucleic acid’ fraction. Aust. J. exp. Biol. med. Sci. 37:353
    [Google Scholar]
  4. Anderson S. G., Ada G. L. 1961; The action of phospholipase A and lipid solvents on Murray Valley encephalitis virus. J. gen. Microbiol. 25:451
    [Google Scholar]
  5. Backus R. C., Williams R. C. 1950; The use of spraying methods and of volatile suspending media in the preparation of specimens for electron microscopy. J. appl. Phys. 21:11–15
    [Google Scholar]
  6. Caraway W. T., Fanger H. 1955; Ultramicro procedures in clinical chemistry. Amer. J. clin. Path. 25:317
    [Google Scholar]
  7. Chargaff E., Zamenhof S. 1948; The isolation of highly polymerised desoxypentose-nucleic acid from yeast cells. J. Mol. Chem. 173:327
    [Google Scholar]
  8. Collins F. D. 1959; Studies on phospholipids. 6. A complex phospholipid from sheep brain. Biochem. J. 72:532
    [Google Scholar]
  9. Donald H. B., Isaacs A. 1954; Counts of influenza virus particles. J. gen. Microbiol. 10:457
    [Google Scholar]
  10. Folch-Pi J. 1955 In Biochemistry of the Developing Nervous System p. 121 Ed. Waelsch H. New York: Academic Press;
    [Google Scholar]
  11. Frommhagen L. H., Freeman N. K., Knight C. A. 1958; The lipid constituents of influenza virus, chick allantoic membrane and sedimentable allantoic protein. Virology 5:173
    [Google Scholar]
  12. Gerould C. H. 1950; Comments on the use of latex spheres as size standards in electron microscopy. J. appl. Phys. 21:183
    [Google Scholar]
  13. Park J. T., Johnson M. J. 1949; A submicro determination of glucose. J. biol. Chem 181:149
    [Google Scholar]
  14. Taylor A. R., Sharp D. G., Beard D., Beard J. W. 1943; Isolation and properties of the equine encephalomyelitis virus (Eastern strain). J. infect. Dis. 73:31
    [Google Scholar]
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