1887

Abstract

SUMMARY: By direct observation of individual organisms in a micro-culture chamber and by viable plate counts, a study has been made of the function of sugar/colloid mixtures in promoting the survival of Gram-negative bacteria on drying. The high death rate after drying in sugar alone was due mainly to cell-wall damage caused during rehydration by the temporary osmotic pressure set up by the sugar within the cell, leading to the formation of spheroplasts not capable of division. Spheroplast formation was largely prevented and survival greatly enhanced by controlled rehydration, showing that the sugar component was the primary protective agent. The complementary role of the protein or ‘protective colloid’ appears to lie in its ability to compress the cell wall against the contracted plasma membrane in plasmolysed cells, thus decreasing the volume of the interspace between the two membranes and so limiting the sugar trapped therein to a safe amount. These observations were corroborated by cell volume measurements in the ultracentrifuge.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-28-4-585
1962-09-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/28/4/mic-28-4-585.html?itemId=/content/journal/micro/10.1099/00221287-28-4-585&mimeType=html&fmt=ahah

References

  1. Annear D. I. 1956; The preservation of bacteria by drying in peptone plugs. J. Hyg., Camb. 54:487
    [Google Scholar]
  2. Annear D. I. 1958; Observations on drying bacteria from the frozen and liquid state. Aust. J. exp. Biol. med. Sci. 36:211
    [Google Scholar]
  3. Bendet I. J., Smith C. E., Lauffer M. A. 1960; Hydrodynamic volumes determined by immiscible liquid displacement. Arch. Biochem. Biophys. 88:280
    [Google Scholar]
  4. Fry R. M. 1954 The preservation of Bacteria in ‘ Biological Applications of Freezing and Drying' Harris R. J. C. New York: Academic Press;
    [Google Scholar]
  5. Fry R. M., Greaves R. I. N. 1951; The survival of bacteria during and after drying. J. Hyg., Camb. 49:220
    [Google Scholar]
  6. Hegarty C. P., Weeks O. B. 1940; Sensitivity of E. coli to cold shock during the logarithmic growth phase. J. Bact. 39:475
    [Google Scholar]
  7. Heller G. 1941; A quantitative study of environmental factors involved in survival and death of bacteria in the desiccated state. J. Bact. 41:109
    [Google Scholar]
  8. Miles A. A., Misra S. S. 1938; Estimation of the bactericidal power of blood. J. Hyg., Camb. 38:732
    [Google Scholar]
  9. Powell E. O. 1956; An improved culture chamber for the study of living bacteria. J. R. micr. Soc. 75:235
    [Google Scholar]
  10. Record B. R., Taylor R. 1953; Some factors influencing the survival of Bact. coli on freeze-drying. J. gen. Microbiol. 9:475
    [Google Scholar]
  11. Scott W. J. 1959; Death during storage of dried micro-organisms. Recent research in freezing and drying Parkes A. S., Smith A. U. Oxford: Blackwell;
    [Google Scholar]
  12. Sharp D. G., Beard D., Beard J. W. 1950; Partial specific volume and water content of influenza virus. J. biol. Chem. 182:279
    [Google Scholar]
  13. Snyder T. L. 1947; The relative errors of bacterial plate-counting methods. J. Bact. 54:641
    [Google Scholar]
  14. Weibull C. 1953; The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme. J. Bact. 66:688
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-28-4-585
Loading
/content/journal/micro/10.1099/00221287-28-4-585
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error