1887

Abstract

SUMMARY: The method of application of negative staining with potassium phosphotungstate for the electron microscopy of bacterial surface structure is described. The method has been used to study the fimbriae of Klebsiella strains, and has confirmed, qualitatively, the results of other workers who used the shadow-casting technique. The diameters of the two types of fimbriae associated with the MS and MR adhesins were found to be 65-70 Å and about 48 Å, respectively. These figures are about 30 % less than earlier estimates. The resistance of both types of fimbriae to prolonged autolysis was shown. Other applications of the negative staining method include the study of bacterial flagellation, and of the structure of cells disintegrated by autolysis or other methods.

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/content/journal/micro/10.1099/00221287-28-1-51
1962-04-01
2021-10-20
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References

  1. Asai T., Okumura S., Tsunoda T. 1957; On the classification of the a-ketoglutaric acid accumulating bacteria in aerobic fermentation. J. gen. appl. Microbiol. 3:13
    [Google Scholar]
  2. Bradley D. E. 1954; Evaporated carbon films for use in electron microscopy. Brit. J. appl. Phys. 5:65
    [Google Scholar]
  3. Brenner S., Horne R. W. 1959; A negative staining method for high resolution electron microscopy of viruses. Biochim. biophys. Acta 34:103
    [Google Scholar]
  4. Brenner S., Streisinger G., Horne R. W., Champe S. P., Barnett L., Benzer S., Rees M. W. 1959; Structural components of bacteriophage. J. mol. Biol. 1:281
    [Google Scholar]
  5. Brinton C. C. 1959; Non-flagellar appendages of bacteria. Nature, Lond. 183:782
    [Google Scholar]
  6. Duguid J. P. 1959; Fimbriae and adhesive properties in Klebsiella strains. J. gen. Microbiol. 21:271
    [Google Scholar]
  7. Duguid J. P., Smith I. W., Dempster G., Edmunds P. N. 1955; Non-flagellar filamentous appendages (‘ Fimbriae’) and haemagglutinating activity in Bacterium coli. J. Path. Bad. 70:335
    [Google Scholar]
  8. Eddy B. P. 1960; Cephalotrichoi s, fermentative, Gram-negative bacteria: the genus Aeromonas. J. appl. Bact. 23:217
    [Google Scholar]
  9. Horne R. W., Waterson A. P., Wildy P., Farnham A. E. 1960; The structure and composition of the Myxoviruses I) Electron microscope studies of the structure of myxovirus particles by negative staining techniques. Virology 11:79
    [Google Scholar]
  10. Houwink A. L. 1949; Discussion in The Nature of the Bacterial Surface. Symp. Soc. gen. Microbiol. 1:92
    [Google Scholar]
  11. Houwink A. L., van Iterson VV. 1950; Electron microscopical observations on bacterial cytology. II. A study ol flagellation. Biochim. biophys. Acta 5:10
    [Google Scholar]
  12. Wildy P., Russell W. C., Horvte R. W. 1960; The morphology of Herpes virus. Virology 12:204
    [Google Scholar]
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