1887

Abstract

Summary: When was grown anaerobically in a complex medium containing -glucose, -ribose or -arginine as energy source the dry wt. of organism produced was proportional to the concentration of the energy source in the medium. However, will not grow in a defined medium with arginine as the energy source unless glucose is present at the same time. The anaerobic growth of both and was proportional to the concentration of glucose in the medium and the yield coefficient—defined as g. dry wt. organism/mole glucose—of the former was the same as that of grown upon glucose and approximately twice that of . Calculation of the g. dry wt. organism/mole adenosine triphosphate synthesized for these three organisms gave values ranging from 12·6 to 8·3 with an average of 10·5. These results suggest that, under anaerobic conditions, the yield of and was proportional to the amount of ATP synthesized. When was grown anaerobically with glucose, glycerol or -lactate as energy source there was, in all three cases, a linear relationship between the dry wt. of organisms produced and the concentration of the energy source in the medium. The values of the yield coefficients obtained were compatible with the formation of approximately 4 mole ATP/mole glucose, 2 mole ATP/mole glycerol and 1 mole ATP/mole lactate.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-23-3-457
1960-12-01
2021-07-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/23/3/mic-23-3-457.html?itemId=/content/journal/micro/10.1099/00221287-23-3-457&mimeType=html&fmt=ahah

References

  1. Akamatsu S., Sekine T. 1951; Hydrolysis of arginine by Streptococcus faecalis . J. biochem. (Japan) 38:349
    [Google Scholar]
  2. Andreasen A.A., Stier J.J.B. 1953; Anaerobic nutrition of Saccharomyces cerevisiae. I. Ergosterol requirement for growth in a defined medium. J. cell. comp. physiol 41:23
    [Google Scholar]
  3. Bauchop T. 1958; Observations on some molar growth yields of bacteria and yeasts. J. gen. Microbiol 18:VII
    [Google Scholar]
  4. Burton R.M. 1957 In methods of enzymology 3246 Colowick S.R., Kaplan N.O. Ed. New York: Academic Press Inc.;
    [Google Scholar]
  5. DeMoss R.D., Bard R.C., Gunsalus I.C. 1951; The mechanism of the heterolactic fermentation: a new route of ethanol formation. J. bact 62:499
    [Google Scholar]
  6. Delwiche E.A. 1950; A biotin function in succinic decarboxylation by PRO pionibacterium pentosaceum. . J. bact 59:439
    [Google Scholar]
  7. Elsden S.R., Gibson Q.H. 1954; The estimation of lactic acid using ceric sulphate. biochem. J 58:154
    [Google Scholar]
  8. Elsden S.R., Peel J.L. 1958; The metabolism of carbohydrates and related compounds. Annu. Rev. Microbiol 12:145
    [Google Scholar]
  9. Fukui S., Ôi A., Ôbayashi A., Kitahara K. 1957; Studies on the pentose metabolism by microorganisms. 1. A new type-lactic acid fermentation of pentose by lactic acid bacteria. J. gen. appl. Microbiol 3:258
    [Google Scholar]
  10. Gibbs M., DeMoss R.D. 1954; Anaerobic dissimilation of C14 labelled glucose and fructose by Pseudomonas lindneri. . J. biol. Chem 7:689
    [Google Scholar]
  11. Gunsalus I.C., Gibbs M. 1952; The heterolactic fermentation. II. Position of C14 in the products of glucose dissimilation by Leuconostoc mesenteraides. . J. biol. Chem 194:871
    [Google Scholar]
  12. Heath E.C., Hurwitz J., Horecker B.L., Ginsberg A. 1958; Pentose fermentation by Lactobacillus plantarum. I. The cleavage xylulose-5-phosphate by phospho-ketolase. J. biol. Chem 131:1009
    [Google Scholar]
  13. Herbert D. 1958; Some principles of continuous culture. In Recent Progress in Microbiology Tunevall G. Ed. Symp. 7th Int. Congr. Microbiol p. 381
    [Google Scholar]
  14. Hurwitz J. 1958; Pentose phosphate cleavage by Leuconostoc mesenteroides. . Biochim. biophys. Acta 28:599
    [Google Scholar]
  15. Knivett V.A. 1954a; Phosphorylation coupled with anaerobic breakdown of citrulline. Biochem. J 56:602
    [Google Scholar]
  16. Knivett V.A. 1954b; The effect of arsenate on bacterial citrulline breakdown. Biochem. J 56:606
    [Google Scholar]
  17. Magasanik B. 1957; Nutrition of bacteria and fungi. Annu. Rev. Microbiol 11:221
    [Google Scholar]
  18. Monod J. 1942 Recherches sur la croissance des cultures bacterienne Paris: Herman et Cie;
    [Google Scholar]
  19. O’dea J.F., Gibbons R.A. 1953; The estimation of small amounts of formaldehyde liberated during the oxidation of carbohydrate and other substances with periodate. Biochem. J 55:580
    [Google Scholar]
  20. Ormerod J.G. 1956; The use of radioactive carbon dioxide in the measurement of carbon dioxide fixation in Rhodospirillum rubrum. . Biochem. J 64:373
    [Google Scholar]
  21. Roberts R.B., Cowie D.B., Abelson P.H., Bolton E., Britten R. 1955; In Studies of Biosynthesis in Escherichia coli. Publ. Cameg. Instn602
    [Google Scholar]
  22. Rosenberger R.F., Elsden S.R. 1960; The yields of Streptococcus faecalis grown in continuous culture. J. gen. Microbiol 22:727
    [Google Scholar]
  23. Slade H.D., Slamp W.C. 1952; The formation of arginine dihydrolase by streptococci and some properties of the enzyme system. J. Bact 64:455
    [Google Scholar]
  24. Smith P.A., Sherman J.H. 1942; The lactic acid fermentation of Streptococci. J. Bact 43:725
    [Google Scholar]
  25. Sokatch J.T., Gunsalus I.C. 1957; Aldonic acid metabolism. I. Pathway of carbon in an inducible gluconate fermentation by Streptococcus faecalis. . J. Bact 73:452
    [Google Scholar]
  26. White J., Munns D.J. 1951; The effect of aeration and other factors on yeast growth and fermentation. Wallerstein Lab. Comm 14:199
    [Google Scholar]
  27. Wood H.G., Stjernholm R., Leaver F.W. 1955; The metabolism of labelled glucose by the propionic acid bacteria. J. Bact 70:510
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-23-3-457
Loading
/content/journal/micro/10.1099/00221287-23-3-457
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error