1887

Abstract

SUMMARY: grown on nutrient broth, casein hydrolysate, or on a mixture of amino acids simulating casein hydrolysate, yielded washed suspensions with an active reductive amination system. On simple defined media the activity of the organisms was relatively low. Additions of a variety of amino acids, singly or in simple mixtures, yielded suspensions with activities up to about half of that attained with complex media. The performance of 18 amino acids in about 200 different combinations was analysed statistically; no amino acid showed marked and significant stimulation of enzyme formation. It was concluded that a high enzyme yield requires a medium with a complex amino acid relationship, and that the performance of complex media cannot be explained directly in terms of a few active components.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-20-1-78
1959-02-01
2024-05-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/20/1/mic-20-1-78.html?itemId=/content/journal/micro/10.1099/00221287-20-1-78&mimeType=html&fmt=ahah

References

  1. Billen D., Lichstein H.C. 1950; Nutritional requirements for hydrogenase production in Escherichia coli. . J. Bact. 60:311
    [Google Scholar]
  2. Billen D., Lichstein H.D. 1951; Nutritional requirements for the production of formic hydrogenlyase, formic dehydrogenase, and hydrogenase in Escherichia coli. . J. Bact. 61:515
    [Google Scholar]
  3. Cohen G.N., Rickenberg H.V. 1956; Concentration specifique reversible des amino acides chez Escherichia coli. . Ann Inst.Pasteur 91:693
    [Google Scholar]
  4. Fairhurst A.S., King H.K., Sewell C.E. 1956; Studies in amino acid biogenesis; the synthesis of alanine from pyruvate and ammonia. J. gen. Microbiol. 15:106
    [Google Scholar]
  5. Gale E.F. 1940; The production of amines by bacteria. 2. The production of tyramine by Streptococcus faecalis. . Biochem. J. 34:846
    [Google Scholar]
  6. Gale E.F., Folkes J.P. 1955; The assimilation of amino acids by bacteria. 21. The effect of nucleic acids in the development of certain enzymic activities in disrupted staphylococcal cells. Biochem. J. 59:675
    [Google Scholar]
  7. Gladstone G.P. 1939; Inter-relationships between amino acids in the nutrition of B. anthracis. . Brit. J. exp. Path. 20:189
    [Google Scholar]
  8. Gordon W.G., Semmett W.F., Cable R.S., Morris M. 1949; The amino- acid composition of α-casein and β-casein. J. Amer. chem. Soc 71:3293
    [Google Scholar]
  9. Pinsky M.J., Stokes J.L. 1952; Requirements for formic hydrogenlyase adaptation in nonproliferating suspensions of Escherichia coli. . J. Bact. 64:151
    [Google Scholar]
  10. Pollock M.R., Wainwright S.D. 1948; Relationship between nitrase and tetrathionase adaptation and cell growth. Brit. J. exp. Path. 29:223
    [Google Scholar]
  11. Sewell C.E. 1954 Amination of Pyruvic acid in Micro-organisms. Thesis University of Liverpool:
    [Google Scholar]
  12. Sewell C.E., King H.K. 1945; A threonine-isoleucine relationship in Bacillus Subtilis. . Biochem. J. 60:xxi
    [Google Scholar]
  13. Shah P.C., King H.K., Hollis B., Fairhurst A.S. 1957; Studies in amino- acid biogenesis: optical activity of amino acids formed by reductive amination in Bacillus Subtilis. . J. gen. Microbiol. 17:620
    [Google Scholar]
  14. Wainwright S.D. 1950; Formation of a bacterial adaptive enzyme system in absence of substrate: production of nitratase by B. COLI without nitrate. Brit. J. exp. Path. 31:495
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-20-1-78
Loading
/content/journal/micro/10.1099/00221287-20-1-78
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error