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SUMMARY: By a cup-plate method, the antibiotic activity of fluid cultures of 253 strains of spore-bearing anaerobic bacilli representing over sixteen Clostridium spp. was tested against one strain each of Mycobacterium phlei, Staphylococcus aureus, and Bacterium coli. Two strains of Clostridium sporogenes and two of Cl. bifermentans showed a weak and variable activity against Mycobacterium phlei. The remainder of the tests were all negative.