A continuous culture apparatus was used to study the effects of air supply and of growth rate on the respiratory activities and the cytochrome content of a strictly aerobic fluorescent pseudomonad. The oxidizing capacity, measured as (succinate), of organisms grown with air as the growth-limiting factor was lower than that of organisms grown with succinate as growth-limiting factor, and with air supply in excess. There was no significant correlation between oxidizing capacity and growth rate under either of the two conditions. Estimation of the cytochrome content of whole organisms grown under these different conditions showed that this tended to decrease with increase in growth rate. Organisms grown with air as the growth-limiting factor always had about double the cytochrome content of cells grown at the same rates but with succinate as the growth-limiting factor.


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