1887

Abstract

SUMMARY: A continuous culture apparatus was used to study the effects of air supply and of growth rate on the respiratory activities and the cytochrome content of a strictly aerobic fluorescent pseudomonad. The oxidizing capacity, measured as Q(succinate), of organisms grown with air as the growth-limiting factor was lower than that of organisms grown with succinate as growth-limiting factor, and with air supply in excess. There was no significant correlation between oxidizing capacity and growth rate under either of the two conditions. Estimation of the cytochrome content of whole organisms grown under these different conditions showed that this tended to decrease with increase in growth rate. Organisms grown with air as the growth-limiting factor always had about double the cytochrome content of cells grown at the same rates but with succinate as the growth-limiting factor.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-19-2-228
1958-10-01
2022-01-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/19/2/mic-19-2-228.html?itemId=/content/journal/micro/10.1099/00221287-19-2-228&mimeType=html&fmt=ahah

References

  1. Baalsrud K., Baalsrud K. S. 1954 Thiobadllus denitrificans. Arch. Mikrobiol 20:34
    [Google Scholar]
  2. Barker H. A., Beck J. V. 1942; Clostridium acidi-uridi and Clostridium cylindrosporum,organisms fermenting uric acid and some other puneris. J. Bact 43:291
    [Google Scholar]
  3. Chaix P., Petit J. F. 1956; Étude des différents spectres cytochromiques de Bacillus subtilis. Biochim. biophys. Acta 22:66
    [Google Scholar]
  4. Chaix P., Petit J. F. 1957; Influence du taux de croissance sur la constitution du spectre hématinique du Bacillus subtilis. Biochim. biophys. Acta 25:481
    [Google Scholar]
  5. Chaix P., Roncoli G. 1950; A propos de la variabilitée du système des cytochromes chez certains microorganismes. Biochim. biophys. Acta 6:268
    [Google Scholar]
  6. Chance B., Williams G. R. 1956; The respiratory chain and oxidative phosphorylation. Advanc. Enzymol 17:65
    [Google Scholar]
  7. Chin C. H. 1950; Effect of aeration on the cytochrome systems of the resting cells of brewers’ yeast. Nature; Lond: 165926
    [Google Scholar]
  8. Cooper M., Fernstrom G. A., Miller S. A. 1944; Performance of agitated gas-liquid extractors. Industr. Engng Chem 36:504
    [Google Scholar]
  9. Ephrussi B., Slonimski P. P. 1951; La synthèse adaptive des cytochromes chez la levure de boulangerie. Biochim. biophys. Acta 6:256
    [Google Scholar]
  10. Herbert D. 1955; Effect of haematin and catalase on growth of Pasteurella pestis from small inocula. 3ème Congrès Internat. Biochim. (Brussels, August 1955) Section 10, Resumé No 4895
    [Google Scholar]
  11. Herbert D., Elsworth R., Telling R. C. 1956; The continuous culture of bacteria; a theoretical and experimental study. J. gen. Microbiol 14:601
    [Google Scholar]
  12. Hollman S., Thofern E. 1955; Über den Einfluss des Sauerstoffes auf die endogene Atmung gramnegativer Darmbakterien. Naturwissenschaften 42:586
    [Google Scholar]
  13. Keilin D., Hartree E. F. 1955; Relationship between certain components of the cytochrome system. Nature; Lond: 176200
    [Google Scholar]
  14. Kogut M. 1952; The formation of α-ketoglutarate from succinate by a strain of Pseudomonas fluorescens. J. gen. Microbiol 7:v
    [Google Scholar]
  15. Kogut M. 1957; Isolation and purification of a soluble cytochrome from a fluorescent Pseudomonad. Biochem. J 65:35P
    [Google Scholar]
  16. Kogut M., Podoski E. P. 1953; Oxidative pathways in a fluorescent Pseudomonas. Biochem. J 55:800
    [Google Scholar]
  17. Krebs H. A. 1937; The rôle of fumarate in the respiration of Bacterium coli commune. Biochem. J 31:2095
    [Google Scholar]
  18. Lenhoff H. M. 1954; Effects of inorganic iron and molybdenum on electron transport in Pseudomonas fluorescens. Fed. Proc 13:828
    [Google Scholar]
  19. Lenhoff H. M., Kaplan N. O. 1953; A cytochrome peroxidase from Pseudomonas fluorescens. Nature; Lond: 172730
    [Google Scholar]
  20. Lenhoff H. M., Nicholas D. J. D., Kaplan N. O. 1956; Effects of oxygen, iron and molybdenum on routes of electron transfer in Pseudomonas fluorescens. J. biol. Chem 220:983
    [Google Scholar]
  21. Longmuir I. S. 1954; Respiration rate of bacteria as a function of oxygen concentration. Biochem. J 57:81
    [Google Scholar]
  22. Markham R. 1942; A steam distillation apparatus suitable for micro-Kjeldahl analysis. Biochem. J 36:790
    [Google Scholar]
  23. Monod J. 1950; La technique de culture continue théorie et applications. Ann. Inst. Pasteur 79:390
    [Google Scholar]
  24. Moss F. 1956; Adaptation of the cytochrome of Aerobacter aerogenes in response to environmental oxygen tension. Aust. J. exp. Biol. med. Sci 39:395
    [Google Scholar]
  25. Novick A., Szilard L. 1950; Experiments with the chemostat on spontaneous mutations of bacteria. Proc. nat. Acad. Sci., Wash 36:708
    [Google Scholar]
  26. Pirt S. J. 1957; The oxygen requirements of growing cultures of an Aerobacter species determined by means of the continuous culture technique. J. gen. Microbiol 16:59
    [Google Scholar]
  27. Sato R., Egami F. 1949; Studies on nitrate reductase. III. Bull. chem. Soc. Japan 22:137
    [Google Scholar]
  28. Shibata K., Benson A. H., Calvin M. 1954; The absorption spectra of suspensions of living micro-organisms. Biochim. biophys. Acta 15:461
    [Google Scholar]
  29. Stanier R. Y., Cohen-Bazire G. 1957; In Microbial Ecology. The rôle of light in the microbial world; some facts and speculations. Symp. Soc. gen. Microbiol 7:56
    [Google Scholar]
  30. Wise W. S. 1951; The measurement of the aeration of culture media. J. gen. Microbiol 5:167
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-19-2-228
Loading
/content/journal/micro/10.1099/00221287-19-2-228
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error