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SUMMARY: Vesicular stomatitis virus was released exponentially and fairly reproducibly after infecting chick embryo cell monolayers. This system appeared suitable for chemical studies, which are to be presented separately; the present paper is concerned with growth-curve methods and a discussion of the system. When the average multiplicity of adsorption was 6 plaque-forming units/cell the latent period was 1–2 hr., and growth appeared to follow a one-step cycle. At least 62–75 % of the cells were capable of releasing virus.
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