1887

Abstract

Enteropathogenic (EPEC) causes severe diarrhoea in young children. The locus of enterocyte effacement (LEE) pathogenicity island comprises a cluster of operons encoding a type III secretion system and related proteins that are associated with EPEC virulence. The operon encodes Ler that positively regulates the , , , and transcriptional units. The LEE operons are repressed at 27 °C and expressed at 37 °C. This paper describes a regulatory cascade of the thermoregulation of LEE operons. including is repressed by H-NS at 27 °C but not at 37 °C. In contrast, the expression of the , , , and transcriptional units is repressed by H-NS at both 27 °C and 37 °C. Upon shifting the culture temperature from 27 °C to 37 °C, Ler is synthesized and in turn activates the expression of , , and by releasing the H-NS mediated repression. In the case of , Ler acts both by alleviating the H-NS mediated repression and by an additional mechanism, as yet to be defined.

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2002-09-01
2020-03-29
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