1887

Abstract

The amino acid sequence of the signal transducer P (GlnB) of the oceanic photosynthetic prokaryote strain PCC 9511 displays a typical cyanobacterial signature and is phylogenetically related to all known cyanobacterial genes, but forms a distinct subclade with two other marine cyanobacteria. P of was not phosphorylated under the conditions tested, despite its highly conserved primary amino acid sequence, including the seryl residue at position 49, the site for the phosphorylation of the protein in the cyanobacterium PCC 7942. Moreover, lacks nitrate and nitrite reductase activities and does not take up nitrate and nitrite. This strain, however, expresses a low- and a high-affinity transport system for inorganic carbon (C; 240 and 4 μM, respectively), a result consistent with the unphosphorylated form of P acting as a sensor for the control of C acquisition, as proposed for the cyanobacterium PCC 6803. The present data are discussed in relation to the genetic information provided by the MED4 genome sequence.

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2002-08-01
2019-10-16
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