1887

Abstract

Knowledge about transport in , an obligate phagolysosomal parasite, is incomplete. The authors investigated the capability of isolated, intact, host-free to transport ribonucleosides while incubated at a pH value typical of lysosomes. Because of the low activities and limitations of obtaining experimental quantities of isolated, purified , incorporation of substrate into nucleic acid was used as a trap for determination of uptake abilities. Virulent wild-type (phase I) organisms possessed uptake capability for all ribonucleosides. Both phase I and phase II (avirulent) organisms incorporated the purine nucleosides guanosine, adenosine and inosine, and showed a more limited uptake of thymidine and uridine. Both phases were poorly active in cytidine uptake. Neither phase of the organism was capable of transport and incorporation of NTPs, CMP, cytosine or uracil. Water space experiments confirmed that the uptake process concentrated the purine nucleosides within the cytoplasm of both wild-type and phase II via a low-pH-dependent mechanism. Comparison of uptake rates in versus verified that the incorporation of ribonucleosides by is a slow process. It is concluded that possesses some transport pathways consistent with utilization of pools of nucleosides found within its host cell lysosomal pathway.

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2002-08-01
2020-01-28
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