1887

Abstract

The gene encoding a haemagglutinin of , , has been identified and the full-length nucleotide sequence determined. A ∼39 kDa protein, recognized by an anti-haemagglutinin monoclonal antibody, mAb4D, was purified from strain 0083 and the N-terminal sequence obtained. The full-length nucleotide sequence was obtained by inverse PCR and the deduced amino acid sequence of the protein encoded was shown to be similar to other outer-membrane proteins of closely related organisms in the HAP group (, , ), especially the P5 protein of . The gene was cloned into a His-tag expression vector and overexpressed in strain M15(pREP4). The identity of the purified recombinant protein as a haemagglutinin was confirmed by haemagglutination of chicken red blood cells and reactivity, in a Western blot, with the monoclonal antibody specific for the serovar A haemagglutinin.

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2002-07-01
2020-09-29
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