1887

Abstract

The gene encoding a haemagglutinin of , , has been identified and the full-length nucleotide sequence determined. A ∼39 kDa protein, recognized by an anti-haemagglutinin monoclonal antibody, mAb4D, was purified from strain 0083 and the N-terminal sequence obtained. The full-length nucleotide sequence was obtained by inverse PCR and the deduced amino acid sequence of the protein encoded was shown to be similar to other outer-membrane proteins of closely related organisms in the HAP group (, , ), especially the P5 protein of . The gene was cloned into a His-tag expression vector and overexpressed in strain M15(pREP4). The identity of the purified recombinant protein as a haemagglutinin was confirmed by haemagglutination of chicken red blood cells and reactivity, in a Western blot, with the monoclonal antibody specific for the serovar A haemagglutinin.

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2002-07-01
2019-12-11
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References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403-410.[CrossRef]
    [Google Scholar]
  2. Altschul, S. F., Madden, T. L., Schaffer, A. J., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25, 3389-3402.[CrossRef]
    [Google Scholar]
  3. Blackall, P. J. & Yamamoto, R. ( 1990; ). Infectious coryza. In A Laboratory Manual for the Isolation and Identification of Avian Pathogens , pp. 27-31. Edited by H. G. Pearson, L. H. Arp, C. H. Domermuth & J. E. Pearson. Ames, IA: American Association of Avian Pathologists.
  4. Blackall, P. J. & Yamamoto, R. (1997). Infectious coryza. In Diseases of Poultry, pp. 179–190. Edited by B. W. B. Calnek, H. J. Barnes, C. W. Beard, L. R. McDougald & Y. M. Saif. Ames, Iowa: Iowa State University Press.
  5. Blackall, P. J., Eaves, L. E. & Aus, G. ( 1990; ). Serotyping of Haemophilus paragallinarum by the Page scheme: comparison of the use of agglutination and hemagglutination-inhibition tests. Avian Dis 34, 643-645.[CrossRef]
    [Google Scholar]
  6. Corpet, F. ( 1988; ). Multiple sequence alignment with hierarchial clustering. Nucleic Acids Res 16, 10881-10890.[CrossRef]
    [Google Scholar]
  7. Dobos, K. M., Khoo, K. H., Swiderek, K. M, Brennan, P. J. & Belisle, J. T. ( 1996; ). Definition of the full extent of glycosylation of the 45-kDa glycoprotein of Mycobacterium tuberculosis. J Bacteriol 178, 2498-2506.
    [Google Scholar]
  8. Dobson, W. J., McCurdy, H. D. & McRae, H. J. ( 1979; ). The function of fimbriae in Myxococcus xanthus. 2. The role of fimbriae in cell–cell interactions. Can J Microbiol 25, 1359-1372.[CrossRef]
    [Google Scholar]
  9. Doig, P. K., Kinsella, N., Guerry, P. & Trust, T. J. ( 1996; ). Characterisation of a post-translational modification of Campylobacter flagellin: identification of a sero-specific glycosyl moiety. Mol Microbiol 19, 379-387.[CrossRef]
    [Google Scholar]
  10. Erickson, P. & Herzberg, M. C. ( 1993; ). Evidence for covalent linkage of a carbohydrate polymer to a glycoprotein of Streptococcus sanguis. J Biol Chem 268, 23780-23783.
    [Google Scholar]
  11. Fleischmann, R. D., Adams, M. D., White, O. & 37 other authors ( 1995; ). Whole genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269, 496–512.[CrossRef]
    [Google Scholar]
  12. Iritani, Y., Katagiri, K. & Arita, H. ( 1980; ). Purification and properties of Haemophilus paragallinarum haemagglutinin. Am J Vet Res 41, 2114-2118.
    [Google Scholar]
  13. Klesney-Tait, J., Hiltke, T. J., Maciver, I., Spinola, S. M., Radolf, J. D. & Hansen, E. J. ( 1997; ). The major outer membrane protein of Haemophilus ducreyi consists of two OmpA homologs. J Bacteriol 179, 1764-1773.
    [Google Scholar]
  14. Komatsuzawa, H., Kawai, T., Wilson, M. E., Taubman, M. A., Sugai, M. & Suginaka, H. ( 1999; ). Cloning of the gene encoding the Actinobacillus actinomycetemcomitans serotype b OmpA-like outer membrane protein. Infect Immun 67, 942-945.
    [Google Scholar]
  15. Kume, K., Sawata, A. & Nakase, Y. ( 1980; ). Relationship between protective activity and antigen structure of Haemophilus paragallinarum serotypes 1 and 2. Am J Vet Res 41, 97-100.
    [Google Scholar]
  16. Kume, K., Sawata, A., Nakai, T. & Matsumoto, M. ( 1983a; ). Serological classification of Haemophilus paragallinarum with a hemagglutinin system. J Clin Microbiol 17, 958-964.
    [Google Scholar]
  17. Kume, K., Sawata, A. & Nakai, T. ( 1983b; ). Serologic and immunologic studies on three types of haemagglutinin of Haemophilus paragallinarum serotype 1 organisms. Jpn J Vet Sci 45, 783-792.[CrossRef]
    [Google Scholar]
  18. Kume, K., Sawata, A. & Nakai, T. ( 1984; ). Clearance of the challenge organisms from the upper respiratory tract of chickens injected with an inactivated Haemophilus paragallinarum vaccine. Jpn J Vet Sci 46, 843-850.[CrossRef]
    [Google Scholar]
  19. Laemmli, U. K. ( 1970; ). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.[CrossRef]
    [Google Scholar]
  20. Maniatis, T. F., Fritsch, E. F. & Sambrook, J. (1989). Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  21. Nielsen, H., Engelbrecht, J., Brunak, S. & von Heijne, G. ( 1997; ). Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng 10, 1-6.[CrossRef]
    [Google Scholar]
  22. Otsuki, K. & Iritani, Y. ( 1974; ). Preparation and immunological response to a new mixed vaccine composed of inactivated Newcastle Disease virus, inactivated infectious bronchitis virus, and inactivated Hemophilus gallinarum. Avian Dis 18, 297-304.[CrossRef]
    [Google Scholar]
  23. Page, L. A. ( 1962; ). Haemophilus infections in chickens: characteristics of 12 Haemophilus isolates recovered from diseased chickens. Am J Vet Res 23, 85-95.
    [Google Scholar]
  24. Power, P. M., Roddam, L. F., Dieckelmann, M., Srikhanta, Y. N., Tan, Y.-C. & Jennings, M. P. ( 2000; ). Genetic characterisation of pilin glycosylation in Neisseria meningititis. Microbiology 146, 967-979.
    [Google Scholar]
  25. Reid, G. G. & Blackall, P. J. ( 1987; ). Comparison of adjuvants for an inactivated infectious coryza vaccine. Avian Dis 31, 59-63.[CrossRef]
    [Google Scholar]
  26. Sawata, A. & Kume, K. ( 1983; ). Relationships between virulence and morphological or serological properties of variants dissociated from serotype 1 Haemophilus paragallinarum strains. J Clin Microbiol 18, 49-55.
    [Google Scholar]
  27. Stimson, E., Virji, M., Makepeace, K. & 9 other authors ( 1995; ). Meningococcal pilin: a glycoprotein substituted with digalactosyl 2,4-diacetoamido-2,4,6-trideoxyhexose. Mol Microbiol 17, 1201–1214.[CrossRef]
    [Google Scholar]
  28. Takagi, M., Hirayama, N., Makie, H. & Ohta, S. ( 1991a; ). Production, characterization and protective effect of monoclonal antibodies to Haemophilus paragallinarum serotype A. Vet Microbiol 27, 327-338.[CrossRef]
    [Google Scholar]
  29. Takagi, M., Ohmae, K., Hirayama, N. & Ohta, S. ( 1991b; ). Expression of hemagglutinin of Haemophilus paragallinarum serotype A in Escherichia coli. J Vet Med Sci 53, 917-920.[CrossRef]
    [Google Scholar]
  30. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). CLUSTALW: improving the sensitivity of progressive multiple alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673-4680.[CrossRef]
    [Google Scholar]
  31. Tuomanen, E. I. ( 1996; ). Surprise? Bacteria glycosylate proteins too. J Clin Invest 98, 2659-2660.[CrossRef]
    [Google Scholar]
  32. Webb, D. C. & Cripps, A. W. ( 1998; ). Secondary structure and molecular analysis of interstrain variability in the P5 outer-membrane protein on non-typable Haemophilus influenzae isolated from diverse anatomical sites. J Med Microbiol 47, 1059-1067.[CrossRef]
    [Google Scholar]
  33. Yamaguchi, T., Kobayashi, M., Masaki, S. & Iritani, Y. ( 1993; ). Isolation and characterization of a Haemophilus paragallinarum mutant that lacks a haemagglutinating antigen. Avian Dis 37, 970-976.[CrossRef]
    [Google Scholar]
  34. Zeng, H., Pandher, K. & Murphy, G. L. ( 1999; ). Molecular cloning of the Pasteurella haemolytica pomA gene and identification of bovine antibodies against PomA surface domains. Infect Immun 67, 4968-4973.
    [Google Scholar]
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