RT Journal Article SR Electronic(1) A1 Hobb, Rhonda I. A1 Tseng, Hsing-Ju A1 Downes, John E. A1 Terry, Tamsin D. A1 Blackall, Patrick J. A1 Takagi, Masami A1 Jennings, Michael P.YR 2002 T1 Molecular analysis of a haemagglutinin of Haemophilus paragallinarumaaThe GenBank accession numbers for the sequences determined in this work are AF491817–AF491827. JF Microbiology, VO 148 IS 7 SP 2171 OP 2179 DO https://doi.org/10.1099/00221287-148-7-2171 PB Microbiology Society, SN 1465-2080, AB The gene encoding a haemagglutinin of H. paragallinarum, hagA, has been identified and the full-length nucleotide sequence determined. A ∼39 kDa protein, recognized by an anti-haemagglutinin monoclonal antibody, mAb4D, was purified from H. paragallinarum strain 0083 and the N-terminal sequence obtained. The full-length nucleotide sequence was obtained by inverse PCR and the deduced amino acid sequence of the protein encoded was shown to be similar to other outer-membrane proteins of closely related organisms in the HAP group (Haemophilus, Actinobacillus, Pasteurella), especially the P5 protein of Haemophilus influenzae. The hagA gene was cloned into a His-tag expression vector and overexpressed in Escherichia coli strain M15(pREP4). The identity of the purified recombinant protein as a H. paragallinarum haemagglutinin was confirmed by haemagglutination of chicken red blood cells and reactivity, in a Western blot, with the monoclonal antibody specific for the serovar A haemagglutinin., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-148-7-2171