1887

Abstract

A small (19·8 kDa) protein was identified in ALKO 2718 cultures during growth in the presence of yeast extract and chitin, but not with glucose. The protein targets β-chitin best, then α-chitin, but barely any other polysaccharide. This described chitin-binding protein (ChbB) is the first of its type from a strain and cross-reacts with antibodies raised against the α-chitin-binding protein CHB1. Using reverse genetics, the chromosomal gene of strain ALKO 2718 was identified, cloned and sequenced. ChbB shares several motifs with the α-chitin-binding proteins CHB1 and CHB2 of and CBP21 of predominantly targeting β-chitin. Synthesis was repressed by glucose and the presence of boxes suggests catabolite control. Using PCR, Southern hybridization and anti-ChbB antibodies, the presence of a gene, as well as of a ChbB protein homologue, was ascertained in several tested strains, but not in 168. Contrary to 168, all strains secreted varying amounts of enzymic activity, degrading carboxymethyl chitin coupled with Remazol brilliant violet.

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2001-07-01
2020-01-27
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