1887

Abstract

Albicidins, a family of potent antibiotics and phytotoxins produced by the sugarcane leaf scald pathogen , inhibit DNA replication in bacteria and plastids. A gene located by Tn-tagging was confirmed by complementation to participate in albicidin biosynthesis. The gene () encodes a large protein (predicted 525695), with a modular architecture indicative of a multifunctional polyketide synthase (PKS) linked to a non-ribosomal peptide synthetase (NRPS). At 4801 amino acids in length, XabB is the largest reported PKS–NRPS. Twelve catalytic domains in this multifunctional enzyme are arranged in the order N terminus–acyl-CoA ligase (AL)–acyl carrier protein (ACP)–β-ketoacyl synthase (KS)–β-ketoacyl reductase (KR)–ACP–ACP–KS–peptidyl carrier protein (PCP)–condensation (C)–adenylation–PCP–C. The modular architecture of XabB indicates likely steps in albicidin biosynthesis and approaches to enhance antibiotic yield. The novel pattern of domains, in comparison with known PKS–NRPS enzymes for antibiotic production, also contributes to the knowledge base for rational design of enzymes producing novel antibiotics.

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2001-03-01
2020-08-12
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