1887

Abstract

Two evolutionary mechanisms have been proposed in the process of protein diversification of the large family of antimicrobial toxins of , known as the colicins. Data from previous studies suggest that the relatively rare nuclease colicins appear to diversify primarily through the action of positive selection, whilst the more abundant pore-former colicins appear to diversify through the action of recombination. The complete DNA sequence of the newly characterized colicin plasmid, pCol-Let, isolated from a Yanomama Indian of South America, is presented here. This plasmid encodes a newly identified pore-former colicin, colicin Y. DNA and protein sequence comparisons of the colicin Y gene cluster and the encoded proteins with those of published pore-former colicins provide the first evidence that positive selection may also act to increase pore-former colicin diversity.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-146-7-1671
2000-07-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/micro/146/7/1461671a.html?itemId=/content/journal/micro/10.1099/00221287-146-7-1671&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403-410.[CrossRef]
    [Google Scholar]
  2. Chan, P. T., Ohmori, H., Tomizawa, J. & Lebowitz, J. ( 1985; ). Nucleotide sequence and gene organization of ColE1 DNA. J Biol Chem 260, 8925-8935.
    [Google Scholar]
  3. Eveland, W. C., Oliver, W. J. & Neel, J. V. ( 1971; ). Characteristics of Escherichia coli serotypes in the Yanomama, a primitive Indian tribe of South America. Infect Immun 4, 753-756.
    [Google Scholar]
  4. Felsenstein, J. ( 1988; ). Phylogenies from molecular sequences: inference and reliability. Annu Rev Genet 22, 521-565.[CrossRef]
    [Google Scholar]
  5. Friedman, D. I., Baumann, M. & Baron, L. S. ( 1976; ). Cooperative effects of bacterial mutations affecting λ N gene expression. Virology 73, 119-127.[CrossRef]
    [Google Scholar]
  6. James, R., Lazdunski, C. & Pattus, F. (1991). Bacteriocins, microcins and lantibiotics. NATO ASI series, vol. 65. New York: Springer.
  7. James, R., Kleanthous, C. & Moore, G. R. ( 1996; ). The biology of E colicins: paradigms and paradoxes. Microbiology 142, 1569-1580.[CrossRef]
    [Google Scholar]
  8. Lau, P. C. K., Parsons, M. & Uchimura, T. (1992). Molecular evolution of E colicin plasmids with emphasis on the endonuclease types. In Bacteriocins, Microcins and Antibiotics. Edited by R. James, C. Lazdunski & F. Pattus. Berlin: Springer.
  9. Miller, H. I. & Friedman, D. I. ( 1980; ). An E. coli gene product required for λ site-specific recombination. Cell 20, 711-719.[CrossRef]
    [Google Scholar]
  10. Morlon, M., Sherratt, D. & Lazdunski, C. ( 1988; ). Identification of functional regions of the colicinogenic plasmid ColA. Mol Gen Genet 211, 223-230.[CrossRef]
    [Google Scholar]
  11. Neel, J. V. ( 1970; ). Lessons from a ‘‘primitive’’ people. Science 170, 815-822.[CrossRef]
    [Google Scholar]
  12. Neel, J. V. (1994). Physician to the Gene Pool. New York: Wiley.
  13. Pilsl, H. & Braun, V. ( 1995; ). Novel colicin 10: assignment of four domains to TonB- and TolC-dependent uptake via the Tsx receptor and to pore formation. Mol Microbiol 16, 57-67.[CrossRef]
    [Google Scholar]
  14. Pugsley, A. P. & Oudega, B. ( 1987; ). Methods for studying colicins and their plasmids. In Plasmids: a Practical Approach, pp. 105-161. Edited by K. G. Hardy. Oxford: IRL Press.
  15. Riley, M. A. ( 1993a; ). Molecular mechanisms of colicin evolution. Mol Biol Evol 10, 1380-1395.
    [Google Scholar]
  16. Riley, M. A. ( 1993b; ). Positive selection for colicin diversity in bacteria. Mol Biol Evol 10, 1048-1059.
    [Google Scholar]
  17. Riley, M. A. ( 1998; ). Molecular mechanisms of bacteriocin evolution. Annu Rev Genet 32, 255-278.[CrossRef]
    [Google Scholar]
  18. Riley, M. A. & Gordon, D. ( 1992; ). A survey of Col plasmids in natural isolates of Escherichia coli and an investigation into the stability of Col-plasmid lineages. J Gen Microbiol 138, 1345-1352.[CrossRef]
    [Google Scholar]
  19. Riley, M. A. & Gordon, D. ( 1995; ). Ecology and evolution of bacteriocins. J Ind Microbiol 17, 155-158.
    [Google Scholar]
  20. Riley, M. A., Tan, Y. & Wang, J. P. ( 1994; ). Nucleotide polymorphism in colicin E1 and Ia plasmids from natural isolates of Escherichia coli. Proc Natl Acad Sci USA 91, 11276-11280.[CrossRef]
    [Google Scholar]
  21. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406-425.
    [Google Scholar]
  22. Smajs, D., Pilsl, H. & Braun, V. ( 1997; ). Colicin U, a novel colicin produced by Shigella boydii. J Bacteriol 179, 4919-4928.
    [Google Scholar]
  23. Swofford, D. (1997). Phylogenetic Analysis using Parsimony (PAUP* 4.0*). Sunderland, MA: Sinauer.
  24. Tan, Y. & Riley, M. A. ( 1997; ). Positive selection and recombination: major molecular mechanisms in colicin diversification. Trends Ecol Evol 12, 348-351.[CrossRef]
    [Google Scholar]
  25. Vuyst, L. D. & Vandamme, E. ( 1994; ). Lactic acid bacteria and bacteriocins: their practical importance. In Bacteriocins of Lactic Acid Bacteria, pp. 1-11. Edited by L. De Vuyst. London: Blackie Academic and Professional.
  26. Yanisch-Perron, C., Vieira, J. & Messing, J. ( 1985; ). Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-146-7-1671
Loading
/content/journal/micro/10.1099/00221287-146-7-1671
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error