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Volume 146, Issue 7

Microvirus of strain Guinea pig Inclusion Conjunctivitis: isolation and molecular characterization

The GenBank accession number for the sequence reported in this paper is U41758.

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Abstract

The authors report the isolation and molecular characterization of a bacteriophage, ϕCPG1, which infects strain Guinea pig Inclusion Conjunctivitis. Purified virion preparations contained isometric particles of 25 nm diameter, superficially similar to spike-less members of the ϕX174 family of bacteriophages. The single-stranded circular DNA genome of ϕCPG1 included five large ORFs, which were similar to ORFs in the genome of a previously described bacteriophage (Chp1) that infects avian . Three of the ORFs encoded polypeptides that were similar to those in a phage infecting the mollicute , a pathogen of honeybees. Lesser sequence similarities were seen between two ORF products and the major capsid protein of the ϕX174 coliphage family and proteins mediating rolling circle replication initiation in phages, phagemids and plasmids. Phage ϕCPG1 is the second member of the genus , the first to infect a member of a species infecting mammals. Similarity searches of the nucleotide sequence further revealed a highly conserved (75% identity) 375 base sequence integrated into the genome of the human pathogen . This genomic segment encodes a truncated 113 residue polypeptide, the sequence of which is 72% identical to the amino-terminal end of the putative replication initiation protein of ϕCPG1. This finding suggests that has been infected by a phage related to ϕCPG1 and that infection resulted in integration of some of the phage genome into the genome.

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  • Accepted:
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  • Published Online:
Keyword(s): bacteriophage , capsid protein , Chlamydia , EB, elementary body , GPIC, Guinea Pig Inclusion Conjunctivitis , MOMP, major outer-membrane protein , RB, reticulate body , RCR, rolling circle replication , Rep protein and Rep, replication initiation protein
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2000-07-01
2024-05-04
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References

  1. Balin B. J., Gerard H. C., Arking E. J., Appelt D. M., Branigan P. J., Abrams J. T., Whittum-Hudson J. A., Hudson A. P. 1998; Identification and localisation of Chlamydia pneumoniae in the Alzheimer’s brain. Med Microbiol Immunol 187:23–42 [CrossRef]
     [Google Scholar]
  2. Chipman P. R., Agbandje-McKenna M., Renaudin J., Baker T. S., McKenna R. 1998; Structural analysis of the spiroplasma virus, SpV4: implications for evolutionary variation to obtain host diversity among the Microviridae. Structure 6:135–145 [CrossRef]
     [Google Scholar]
  3. Ebisu S., Murahashi Y., Takagi H., Kadowaki K., Yamaguchi K., Yamagata H., Udaka S. 1995; Nucleotide sequence and replication properties of the Bacillus borstelensis cryptic plasmid pHT926. Appl Environ Microbiol 61:3154–3157
     [Google Scholar]
  4. Grayston J. T., Aldous M. B., Easton A., Wang S.-P., Kuo C.-C., Campbell L. A., Altman J. 1993; Evidence that Chlamydia pneumoniae causes pneumonia and bronchitis. J Infect Dis 168:1231–1235 [CrossRef]
     [Google Scholar]
  5. Harshbarger J. C., Chang S. C., Otto S. V. 1977; Chlamydiae (with phages), mycoplasmas, and rickettsiae in Chesapeake Bay bivalves. Science 196:666–668 [CrossRef]
     [Google Scholar]
  6. Hayashi M., Aoyama A., Richardson D. L. Jr, Hayashi M. N. 1988; Biology of the Bacteriophage ϕ X174. In The BacteriophagesThe Viruses vol. 2 pp. 1–71Edited by Calendar R. New York: Plenum;
     [Google Scholar]
  7. Hendrix R. W., Smith M. C., Burns R. N., Ford M. E., Hatfull G. F. 1999; Evolutionary relationships among diverse bacteriophages and prophages: all the world’s a phage. Proc Natl Acad Sci USA 96:2192–2197 [CrossRef]
     [Google Scholar]
  8. Hsia R.-c., Ohayon H., Gounon P., Dautry-Varsat A., Bavoil P. M. 2000; Phage infection of the obligate intracellular bacterium, Chlamydia psittaci strain Guinea Pig Inclusion Conjunctivitis. Microbes Infect (in press)
    [Google Scholar]
  9. Hyman C. L., Augenbraun M. H., Roblin P. M., Schachter J., Hammerschlag M. R. 1991; Asymptomatic respiratory tract infection with Chlamydia pneumoniae TWAR. J Clin Microbiol 29:2082–2083
     [Google Scholar]
  10. Ilyina T. V., Koonin E. V. 1992; Conserved sequence motifs in the initiator proteins for rolling circle DNA replication encoded by diverse replicons from eubacteria, eucaryotes and archaebacteria. Nucleic Acids Res 20:3279–3285 [CrossRef]
     [Google Scholar]
  11. Kalman S., Mitchell W., Marathe R., Lammel C., Fan J., Hyman R. W., Olinger L., Grimwood J., Davis R. W., Stephens R. S. 1999; Comparative genomes of Chlamydia pneumoniae and Chlamydia trachomatis. Nat Genet 21:385–389 [CrossRef]
     [Google Scholar]
  12. Kodaira K., Nakano K., Okada S., Taketo A. 1992; Nucleotide sequence of the genome of the bacteriophage α3: interrelationship of the genome structure and the gene products with those of the phages, ϕX174, G4 and ϕK. Biochim Biophys Acta 1130:277–288 [CrossRef]
     [Google Scholar]
  13. Kuo C.-C., Shor A., Campbell L. A., Fukushi H., Patton D. L., Grayston J. T. 1993; Demonstration of Chlamydia pneumoniae in atherosclerotic lesions of coronary arteries. J Infect Dis 167:841–849 [CrossRef]
     [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680–685 [CrossRef]
     [Google Scholar]
  15. Laurila A., Anttila T., Laara E., Bloigu A., Virtamo J., Albanes D., Lainonen M., Saikku P. 1997; Serological evidence of an association between Chlamydia pneumoniae infection and lung cancer. Int J Cancer 74:31–34 [CrossRef]
     [Google Scholar]
  16. Moulder J. W. 1988; Characteristics of Chlamydiae. In Microbiology of Chlamydia pp. 3–19Edited by Barron A. L. Boca Raton, FL: CRC Press;
     [Google Scholar]
  17. Moulder J. W., Hatch T. P., Kuo C.-C., Schachter J., Storz J. 1984; Chlamydia. In Bergey’s Manual of Systematic Bacteriology pp. 729–739Edited by Krieg N. R., Holt J. G. Baltimore, MD: Williams & Wilkins;
     [Google Scholar]
  18. Renaudin J., Pascarel M.-C., Bové J.-M. 1987; Spiroplasma virus 4: nucleotide sequence of the viral DNA, regulatory signals, and proposed genome organisation. J Bacteriol 169:4950–4961
     [Google Scholar]
  19. Richmond S. J., Stirling P., Ashley C. R. 1982; Virus infecting the reticulate bodies of an avian strain of Chlamydia psittaci. FEMS Microbiol Lett 14:31–36 [CrossRef]
     [Google Scholar]
  20. Richmond S., Storey C., Lusher M. 1990; Transmission of phage Chp1 within Chlamydia. In Chlamydial Infections pp. 153–156Edited by Bowie W. R.others Cambridge, UK: Cambridge University Press;
     [Google Scholar]
  21. Saikku P., Leinonen M., Tenkanen L., Linnanmaki E., Ekman M. R., Manninen V., Manttari M., Frick M. H., Huttunen J. K. 1992; Chronic Chlamydia pneumoniae infection as a risk factor for coronary heart disease in the Helsinki Heart Study. Ann Intern Med 116:273–278 [CrossRef]
     [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467 [CrossRef]
     [Google Scholar]
  23. Schachter J. 1988; Overview of human diseases. In Microbiology of Chlamydia pp. 153–166Edited by Barron A. L. Boca Raton, FL: CRC Press;
     [Google Scholar]
  24. Schachter J., Wyrick P. B. 1994; Culture and isolation of Chlamydia trachomatis. Methods Enzymol 236:377–390
     [Google Scholar]
  25. Stephens R. S., Kalman S., Lammel C.9 other authors 1998; Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis. Science 282:754–759 [CrossRef]
     [Google Scholar]
  26. Storey C. C., Lusher M., Richmond S. J. 1989a; Analysis of the complete nucleotide sequence of Chp1, a phage which infects avian Chlamydia psittaci. J Gen Virol 70:3381–3390 [CrossRef]
     [Google Scholar]
  27. Storey C. C., Lusher M., Richmond S. J., Bacon J. 1989b; Further characterisation of a bacteriophage recovered from an avian strain of Chlamydia psittaci. J Gen Virol 70:1321–1327 [CrossRef]
     [Google Scholar]
  28. Storz J. 1988; Overview of animal diseases induced by chlamydial infection. In Microbiology of Chlamydia pp. 167–192Edited by Barron A. L. Boca Raton, FL: CRC Press;
     [Google Scholar]
  29. Strathmann M., Hamilton B. A., Mayeda C. A., Simon M. I., Meyerowitz E. M., Palazzolo M. J. 1991; Transposon-facilitated DNA sequencing. Proc Natl Acad Sci USA 88:1247–1250 [CrossRef]
     [Google Scholar]
  30. Wyrick P. B., Choong J., Davis C. H., Knight S. T., Royal M. O., Maslow A. S., Bagnell C. R. 1989; Entry of genital Chlamydia trachomatis into polarised human epithelial cells. Infect Immun 57:2378–2389
     [Google Scholar]
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Microvirus of Chlamydia psittaci strain Guinea pig Inclusion Conjunctivitis: isolation and molecular characterizationThe GenBank accession number for the sequence reported in this paper is U41758.
Microbiology 146, 1651 (2000); https://doi.org/10.1099/00221287-146-7-1651
/content/journal/micro/10.1099/00221287-146-7-1651
/content/journal/micro/10.1099/00221287-146-7-1651
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