1887

Abstract

The pathogenesis of is associated with expression of virulence factors, many of which are controlled by two -acylhomoserine lactone (AHL)-based quorum-sensing systems. strains equipped with a -based monitor system expressing green fluorescent protein (GFP) in the presence of exogenous AHL molecules were used to detect the production of AHLs from . Mice were challenged intratracheally with alginate beads containing and and killed on different days after the challenge. By means of confocal scanning laser microscopy, GFP-expressing bacteria could be detected in the lung tissues, indicating production and excretion of AHL molecules by the infecting . AHL signals were detected mainly in lung tissues exhibiting severe pathological changes. These findings support the view that expression of AHL molecules by during infection coincides with its pathogenesis.

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2000-10-01
2020-09-20
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