1887

Abstract

The pathogenesis of is associated with expression of virulence factors, many of which are controlled by two -acylhomoserine lactone (AHL)-based quorum-sensing systems. strains equipped with a -based monitor system expressing green fluorescent protein (GFP) in the presence of exogenous AHL molecules were used to detect the production of AHLs from . Mice were challenged intratracheally with alginate beads containing and and killed on different days after the challenge. By means of confocal scanning laser microscopy, GFP-expressing bacteria could be detected in the lung tissues, indicating production and excretion of AHL molecules by the infecting . AHL signals were detected mainly in lung tissues exhibiting severe pathological changes. These findings support the view that expression of AHL molecules by during infection coincides with its pathogenesis.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-146-10-2481
2000-10-01
2019-11-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/146/10/1462481a.html?itemId=/content/journal/micro/10.1099/00221287-146-10-2481&mimeType=html&fmt=ahah

References

  1. Andersen, J. B., Sternberg, C., Poulsen, L., Bjorn, S., Givskov, M. & Molin, S. ( 1998; ). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl Environ Microbiol 64, 2240-2246.
    [Google Scholar]
  2. Baltimore, R. S., Christie, C. D. & Smith, G. J. ( 1989; ). Immunohistopathologic localization of Pseudomonas aeruginosa in lungs from patients with cystic fibrosis. Implications for the pathogenesis of progressive lung deterioration. Am Rev Respir Dis 140, 1650-1661.[CrossRef]
    [Google Scholar]
  3. Bergen, G. & Shelhamer, J. ( 1996; ). Pulmonary infiltrates in the cancer patient. New approaches to an old problem. Infect Dis Clin N Am 10, 297-325.[CrossRef]
    [Google Scholar]
  4. Bowton, D. ( 1999; ). Nosocomial pneumonia in the ICU – year 2000 and beyond. Chest 115, 28S-33S.[CrossRef]
    [Google Scholar]
  5. Brint, J. M. & Ohman, D. E. ( 1995; ). Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of RhlR-RhlI, another set of regulators in strain PAO1 with homology to the autoinducer-responsive LuxR-LuxI family. J Bacteriol 177, 7155-7163.
    [Google Scholar]
  6. Chapon-Herve, V., Akrim, M., Latifi, A., Williams, P., Lazdunski, A. & Bally, M. ( 1997; ). Regulation of the xcp secretion pathway by multiple quorum-sensing modulons in Pseudomonas aeruginosa. Mol Microbiol 24, 1169-1178.[CrossRef]
    [Google Scholar]
  7. Cormack, B. P., Valdivia, R. H. & Falkow, S. ( 1996; ). FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173, 33-38.[CrossRef]
    [Google Scholar]
  8. Costerton, J. W., Cheng, K. J., Geesey, G. G., Ladd, T. I., Nickel, J. C., Dasgupta, M. & Marrie, T. J. ( 1987; ). Bacterial biofilms in nature and disease. Annu Rev Microbiol 41, 435-464.[CrossRef]
    [Google Scholar]
  9. Davies, D., Parsek, M., Pearson, J., Iglewski, B., Costerton, J. & Greenberg, E. ( 1998; ). The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science 280, 295-298.[CrossRef]
    [Google Scholar]
  10. Finch, R., Pritchard, D., Bycroft, B., Williams, P. & Stewart, G. ( 1998; ). Quorum sensing: a novel target for anti-infective therapy. J Antimicrob Chemother 42, 569-571.[CrossRef]
    [Google Scholar]
  11. Fuqua, C. & Greenberg, E. ( 1998; ). Self perception in bacteria: quorum sensing with acylated homoserine lactones. Curr Opin Microbiol 1, 183-189.[CrossRef]
    [Google Scholar]
  12. Fuqua, C., Winans, S. & Greenberg, E. ( 1996; ). Census and consensus in bacterial ecosystems: the LuxR-LuxI family of quorum-sensing transcriptional regulators. Annu Rev Microbiol 50, 727-751.[CrossRef]
    [Google Scholar]
  13. Gambello, M. J. & Iglewski, B. H. ( 1991; ). Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression. J Bacteriol 173, 3000-3009.
    [Google Scholar]
  14. Gambello, M. J., Kaye, S. & Iglewski, B. H. ( 1993; ). LasR of Pseudomonas aeruginosa is a transcriptional activator of the alkaline protease gene (apr) and an enhancer of exotoxin A expression. Infect Immun 61, 1180-1184.
    [Google Scholar]
  15. Geisenberger, O., Givskov, M., Riedel, K., Høiby, N., Tümmler, B. & Eberl, L. ( 2000; ). Production of N-acyl-l-homoserine lactones by Pseudomonas aeruginosa isolates from chronic lung infections associated with cystic fibrosis. FEMS Microbiol Lett 184, 273-277.
    [Google Scholar]
  16. Givskov, M., de-Nys, R., Manefield, M., Gram, L., Maximilien, R., Eberl, L., Molin, S., Steinberg, P. D. & Kjelleberg, S. ( 1996; ). Eukaryotic interference with homoserine lactone mediated procaryotic signalling. J Bacteriol 178, 6618-6622.
    [Google Scholar]
  17. Glessner, A., Smith, R., Iglewski, B. & Robinson, J. ( 1999; ). Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of twitching motility. J Bacteriol 181, 1623-1629.
    [Google Scholar]
  18. Govan, J. R. & Deretic, V. ( 1996; ). Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia. Microbiol Rev 60, 539-574.
    [Google Scholar]
  19. Hardman, A., Stewart, G. & Williams, P. ( 1998; ). Quorum sensing and the cell-cell communication dependent regulation of gene expression in pathogenic and non-pathogenic bacteria. Antonie Leeuwenhoek 74, 199-210.[CrossRef]
    [Google Scholar]
  20. Hardman, A., Rodgers, H. C., Middleton, B., Camara, M., Knox, A. J. & Williams, P. (1999). Detection of N-acylhomoserine lactones in CF sputum – a new marker of inflammation in cystic fibrosis. Pediat Pulmonol (suppl.) 19, 274.
  21. Hartman, G. & Wise, R. ( 1998; ). Quorum sensing: potential means of treating gram-negative infection? Lancet 351, 848-849.[CrossRef]
    [Google Scholar]
  22. Hastings, J. & Greenberg, E. ( 1999; ). Quorum sensing: the explanation of a curious phenomenon reveals a common characteristic of bacteria. J Bacteriol 181, 2667-2668.
    [Google Scholar]
  23. Hoiby, N. ( 1995; ). Microbiology of cystic fibrosis. In Cystic Fibrosis , pp. 75-98. Edited by M. E. Hodson & D. M. Geddes. London:Chapman & Hall.
  24. Hoiby, N., Giwercman, B., Jensen, T., Johansen, H. K., Kronborg, G., Pressler, T. & Kharazmi, A. (1993). Immune response in cystic fibrosis – helpful or harmful? In Clinical Ecology of Cystic Fibrosis, pp. 133–141. Edited by H. Scobar and others. Amsterdam: Excerpta Medica.
  25. Holloway, B. W. & Morgan, A. F. ( 1986; ). Genome organization in Pseudomonas. Annu Rev Microbiol 40, 79-105.[CrossRef]
    [Google Scholar]
  26. Jensen, E. T., Kharazmi, A., Garred, P., Kronborg, G., Fomsgaard, A., Mollnes, T. E. & Hoiby, N. ( 1993; ). Complement activation by Pseudomonas aeruginosa biofilms. Microb Pathog 15, 377-388.[CrossRef]
    [Google Scholar]
  27. Johansen, H. K., Espersen, F., Pedersen, S. S., Hougen, H. P., Rygaard, J. & Hoiby, N. ( 1993; ). Chronic Pseudomonas aeruginosa lung infection in normal and athymic rats. APMIS 101, 207-225.[CrossRef]
    [Google Scholar]
  28. Jones, S., Yu, B., Bainton, N. J. & 11 other authors ( 1993; ). The lux autoinducer regulates the production of exoenzyme virulence determinants in Erwinia carotovora and Pseudomonas aeruginosa. EMBO J 12, 2477–2482.
    [Google Scholar]
  29. Kharazmi, A., Schitz, P. O., Hoiby, N., Baek, L. & Doring, G. ( 1986; ). Demonstration of neutrophil chemotactic activity in the sputum of cystic fibrosis patients with Pseudomonas aeruginosa infection. Eur J Clin Invest 16, 143-148.[CrossRef]
    [Google Scholar]
  30. Koch, C. & Hoiby, N. ( 1993; ). Pathogenesis of cystic fibrosis. Lancet 341, 1065-1069.[CrossRef]
    [Google Scholar]
  31. Latifi, A., Foglino, M., Tanaka, K., Williams, P. & Lazdunski, A. ( 1996; ). A hierarchical quorum-sensing cascade in Pseudomonas aeruginosa links the transcriptional activators LasR and RhIR (VsmR) to expression of the stationary-phase sigma factor RpoS. Mol Microbiol 21, 1137-1146.[CrossRef]
    [Google Scholar]
  32. Manefield, M., De Nys, R., Kumar, N., Read, R., Givskov, M., Steinberg, P. & Kjellebjerg, S. ( 1999; ). Evidence that halogenated furanones from Delisea pulchra inhibit acylated homoserine lactone (AHL)-mediated gene expression by displacing the AHL signal from its receptor protein. Microbiology 145, 283-291.[CrossRef]
    [Google Scholar]
  33. Moser, C., Johansen, H. K., Song, Z. J., Hougen, H. P., Rygaard, J. & Hoiby, N. ( 1997; ). Chronic Pseudomonas aeruginosa lung infection is more severe in Th2 responding BALB/c mice compared to Th1 responding C3H/HeN mice. APMIS 105, 838-842.[CrossRef]
    [Google Scholar]
  34. Ochsner, U. A. & Reiser, J. ( 1995; ). Autoinducer-mediated regulation of rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A 92, 6424-6428.[CrossRef]
    [Google Scholar]
  35. Passador, L., Cook, J. M., Gambello, M. J., Rust, L. & Iglewski, B. H. ( 1993; ). Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication. Science 260, 1127-1130.[CrossRef]
    [Google Scholar]
  36. Pearson, J. P., Gray, K. M., Passador, L., Tucker, K. D., Eberhard, A., Iglewski, B. H. & Greenberg, E. P. ( 1994; ). Structure of the autoinducer required for the expression of Pseudomonas aeruginosa virulence genes. Proc Natl Acad Sci U S A 91, 197-201.[CrossRef]
    [Google Scholar]
  37. Pearson, J. P., Passador, L., Iglewski, B. H. & Greenberg, E. P. ( 1995; ). A second N-acylhomoserine lactone signal produced by Pseudomonas aeruginosa. Proc Natl Acad Sci U S A 92, 1490-1494.[CrossRef]
    [Google Scholar]
  38. Pearson, J., Pesci, E. & Iglewski, B. ( 1997; ). Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes. J Bacteriol 179, 5756-5767.
    [Google Scholar]
  39. Pearson, J., Van Delden, C. & Iglewski, B. ( 1999; ). Active efflux and diffusion are involved in transport of Pseudomonas aeruginosa cell-to-cell signals. J Bacteriol 181, 1203-1210.
    [Google Scholar]
  40. Pedersen, S. S., Shand, G. H., Hansen, B. L. & Hansen, G. N. ( 1990; ). Induction of experimental chronic Pseudomonas aeruginosa lung infection with P. aeruginosa entrapped in alginate microspheres. APMIS 98, 203-211.[CrossRef]
    [Google Scholar]
  41. Pesci, E., Pearson, J., Seed, P. & Iglewski, B. ( 1997; ). Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa. J Bacteriol 179, 3127-3132.
    [Google Scholar]
  42. Pirhonen, M., Flego, D., Heikinheimo, R. & Palva, E. T. ( 1993; ). A small diffusible signal molecule is responsible for the global control of virulence and exoenzyme production in the plant pathogen Erwinia carotovora. EMBO J 12, 2467-2476.
    [Google Scholar]
  43. Richard, P., Le Floch, R., Chamoux, C., Pannier, M., Espaze, E. & Richet, H. ( 1994; ). Pseudomonas aeruginosa outbreak in a burn unit: role of antimicrobials in the emergence of multiply resistant strains. J Infect Dis 170, 377-383.[CrossRef]
    [Google Scholar]
  44. Salmond, G. P., Bycroft, B. W., Stewart, G. S. & Williams, P. ( 1995; ). The bacterial ‘enigma’: cracking the code of cell–cell communication. Mol Microbiol 16, 615-624.[CrossRef]
    [Google Scholar]
  45. Seed, P. C., Passador, L. & Iglewski, B. H. ( 1995; ). Activation of the Pseudomonas aeruginosa lasI gene by LasR and the Pseudomonas autoinducer PAI: an autoinduction regulatory hierarchy. J Bacteriol 177, 654-659.
    [Google Scholar]
  46. Shaw, P., Ping, G., Daly, S., Cha, C., Cronan, J. J., Rinehart, K. & Farrand, S. ( 1997; ). Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography. Proc Natl Acad Sci USA 94, 6036-6041.[CrossRef]
    [Google Scholar]
  47. Song, Z., Kharazmi, A., Wu, H., Faber, V., Moser, C., Johansen, H. K., Rygaard, J. & Hoiby, N. ( 1998; ). Effects of ginseng treatment on neutrophil chemiluminescence and immunoglobulin G subclasses in a rat model of chronic Pseudomonas aeruginosa pneumonia. Clin Diagn Lab Immunol 5, 882-887.
    [Google Scholar]
  48. Swift, S., Karlyshev, A., Fish, L., Durant, E., Winson, M., Chhabra, S., Williams, P., Macintyre, S. & Stewart, G. ( 1997; ). Quorum sensing in Aeromonas hydrophila and Aeromonas salmonicida: identification of the LuxRI homologs AhyRI and AsaRI and their cognate N-acylhomoserine lactone signal molecules. J Bacteriol 179, 5271-5281.
    [Google Scholar]
  49. Tang, H. B., DiMango, D., Bryan, R., Gambello, M., Iglewski, B. H., Goldberg, J. B. & Prince, A. ( 1996; ). Contribution of specific Pseudomonas aeruginosa virulence factors to pathogenesis of pneumonia in a neonatal mouse model of infection. Infect Immun 64, 37-43.
    [Google Scholar]
  50. Telford, G., Wheeler, D., Williams, P., Tomkins, P. T., Appleby, P., Sewell, H., Stewart, G. S. A. B., Bycroft, B. W. & Pritchard, D. I. ( 1998; ). The Pseudomonas aeruginosa quorum-sensing signal molecule N-(3-oxododecanoyl)-l-homoserine lactone has immunomodulatory activity. Infect Immun 66, 36-42.
    [Google Scholar]
  51. Van Delden, C. & Iglewski, B. ( 1998; ). Cell-to-cell signaling and Pseudomonas aeruginosa infections. Emerg Infect Dis 4, 551-560.[CrossRef]
    [Google Scholar]
  52. Winson, M., Camara, M., Latifi, A. & 10 other authors ( 1995; ). Multiple N-acyl-l-homoserine lactone signal molecules regulate production of virulence determinants and secondary metabolites in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A 92, 9427–9431.[CrossRef]
    [Google Scholar]
  53. Yanisch-Perron, C., Vieira, J. & Messing, J. ( 1985; ). Improved M13 cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-146-10-2481
Loading
/content/journal/micro/10.1099/00221287-146-10-2481
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error